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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 04, 2018 |
| Title |
Transcription factor activity and nucleosome organisation in mitosis |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
Mitotic bookmarking transcription factors (BFs) maintain the capacity to bind to their targets during mitosis, despite major rearrangements of the chromatin. While they were thought to propagate gene regulatory information through mitosis by statically occupying their DNA targets, it has recently become clear that BFs are highly dynamic in mitotic cells. This represents both a technical and a conceptual challenge to study and understand the function of BFs: first, formaldehyde has been suggested to be unable to efficiently capture these transient interactions, leading to profound contradictions in the literature; second, if BFs are not permanently bound to their targets during mitosis, it becomes unclear how they convey regulatory information to daughter cells. Here, comparing formaldehyde to alternative fixatives we clarify the nature of the chromosomal association of previously proposed BFs in embryonic stem cells: while ESRRB can be considered as a canonical BF that binds at selected regulatory regions in mitosis, SOX2 and OCT4 establish DNA sequence independent interactions with the mitotic chromosomes, either throughout the chromosomal arms (SOX2) or at pericentromeric regions (OCT4). Moreover, we show that ordered nucleosomal arrays are retained during mitosis at ESRRB bookmarked sites, whereas regions losing transcription factor binding display a profound loss of order. By maintaining nucleosome positioning during mitosis, ESRRB might ensure the rapid post-mitotic re-establishment of functional regulatory complexes at selected enhancers and promoters. Our results provide a mechanistic framework that reconciles dynamic mitotic binding with the transmission of gene regulatory information across cell division.
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| Overall design |
Examination of binding of Esrrb, Nanog, Sox2 and Oct4 in mouse ES cells in interphase and mitosis by ChIP-seq with differing fixatives: FA and DSG+FA. Examination of chromatin accessibility and nucleosome positioning in mouse ES cells in interphase and mitosis by ATAC-seq and MNase-seq. Examination of the consequences of potential Sox2 bookmarking by cell-cycle resolved RNA-seq of Sox2-AID line.
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| Contributor(s) |
Owens N, Navarro P |
| Citation(s) |
30655337 |
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| Submission date |
Nov 15, 2018 |
| Last update date |
Jun 16, 2020 |
| Contact name |
Pablo Navarro |
| E-mail(s) |
pnavarro@pasteur.fr
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| Phone |
+33145688285
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| Organization name |
Institut Pasteur
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| Street address |
25 rue du Docteur Roux
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| City |
paris |
| ZIP/Postal code |
75015 |
| Country |
France |
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| Platforms (4)
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| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (97)
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| Relations |
| BioProject |
PRJNA505682 |
| SRA |
SRP169520 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE122589_RAW.tar |
10.3 Gb |
(http)(custom) |
TAR (of BW) |
| GSE122589_mnase_atac_meta_plot_library.tsv.gz |
3.1 Mb |
(ftp)(http) |
TSV |
| GSE122589_sox2_raw_counts.tsv.gz |
860.6 Kb |
(ftp)(http) |
TSV |
| GSE122589_sox2_tpm.tsv.gz |
846.9 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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