CUT and RUN was performed for 3 NK populations and found CD94+CD56hi NK cells exhibit unique H3K4me1, H3K4me4 enriched region, we also identified TCF7 binding region in CD94+CD56hi NK cells.
Overall design
CD94-CD56dim, CD94+CD56dim and CD94+CD56hi NK cells were sorted then subject to CUT and RUN using antibodies against rabbit IgG, H3K4me1, H3K4me3, H3K27ac, H3K27me3, TCF7
A note on the file naming convention: - "sample1" or "sample2" represents 2 different donors; - "minus" represents CD94-CD56dim NK cells, "plus" represents CD94+CD56dim NK cells, "plusplus" represents CD94+CD56hi NK cells. - The last digit "0-5" represents different antibodies used in CUT&RUN: "0" represents Rabbit anti-human IgG "1" represents anti-H3K4me1 "2" represents anti-H3K27ac "3" represents anti-H3K4me3 "4" represents anti-TCF7 "5" represents anti-H3K27me3
Example: GSM3473668 "cut and run sample1 minus0" in GEO data set means Rabbit anti-human IgG CUT&RUN for CD94-CD56dim NK cells from donor 1.
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