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Series GSE12248 Query DataSets for GSE12248
Status Public on Dec 31, 2008
Title Genetic architecture of murine skin inflammation and tumor susceptibility
Platform organism Mus musculus
Sample organisms Mus musculus; Mus spretus; Mus musculus x Mus spretus
Experiment type Expression profiling by array
Summary Gene expression in self-renewing epithelial tissues is controlled by cis- and trans-activating regulatory factors that mediate responses to exogenous agents capable of causing tissue damage, infection, inflammation, or tumorigenesis. We used network construction methods to analyze the genetic architecture of gene expression in normal mouse skin in a cross between tumor-susceptible Mus musculus and tumor-resistant Mus spretus. We demonstrate that gene expression motifs representing different constituent cell types within the skin such as hair follicle cells, haematopoietic cells, and melanocytes are under separate genetic control. Motifs associated with inflammation, epidermal barrier function and proliferation are differentially regulated in mice susceptible or resistant to tumor development. The intestinal stem cell marker Lgr5 is identified as a candidate master regulator of hair follicle gene expression, and the Vitamin D receptor (Vdr) links epidermal barrier function, inflammation, and tumor susceptibility.

Keywords: Expression Quantitative Trait Loci
Overall design A backcross was generated using male Mus spretus and female FVB/N mice; female F1 hybrids were mated with male FVB/N mice. Seventy-one backcross mice (8-12 weeks old) received a single dose of DMBA (25 µg per mouse in 200 µl acetone). Starting one week after the initiation tumors were promoted with TPA (200 µl of 10-4 M solution in acetone) twice weekly for 20 weeks. Initiation and promotion were performed on doral back skin. Normal tail skin was snap frozen when the animals were sacrificed. Tail epidermis from completely untreated Spretus, FVB, and Spretus x FVB F1 mice was also analyzed.
Contributor(s) Quigley D, To MD, Pérez-Losada J, Mao J, Nagase H, Ginzinger DG, Balmain A
Citation(s) 19136944, 24785298
Submission date Jul 25, 2008
Last update date Feb 11, 2019
Contact name David Quigley
Organization name UCSF
Department Helen Diller Comprehensive Cancer Center
Lab Ashworth Lab
Street address 1450 Third St. Room 207
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (83)
GSM307750 Tail-FVBBX-1
GSM307751 Tail-FVBBX-2
GSM307752 Tail-FVBBX-3
This SubSeries is part of SuperSeries:
GSE21264 Inflammation and tumor susceptibility in skin cancer
BioProject PRJNA129003

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12248_RAW.tar 273.9 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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