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Series GSE122474 Query DataSets for GSE122474
Status Public on Dec 31, 2019
Title Transcriptome analysis of bovine uninucleated trophoblast cells (UTC) and Trophoblast Giant Cells (TGC)
Organism Bos taurus
Experiment type Expression profiling by array
Summary In the bovine placenta, intimate fetomaternal contacts are confined to multiple discrete structures termed placentomes. In the placentomes, widely ramified fetal chorionic villi interdigitate with corresponding maternal caruncular crypts. The trophoblast covering the chorionic villi is composed of uninucleate trophoblast cells (UTCs) and binucleate trophoblast giant cells (TGCs). TGCs are weakly invasive and capable of migrating toward the caruncular epithelium where they eventually fuse with single epithelial cells to form short-living trinucleate fetomaternal hybrid cells. Thereby, molecules of fetal origin are transported into the maternal compartment. The loss of TGCs due to fetomaternal hybrid cell formation is compensated by differentiation of UTCs into TGCs. We performed a genome-wide gene expression study of virtually pure UTCs and TGCs to to identify genes which are associated with trophoblast cell differentiation
Overall design Bovine placentomes were obtained from three pregnant cows from days 118 to 130 of gestation. UTCs and TGCs were isolated by an optimized fluorescence activated cell sorting (FACS) procedure. RNA was isolated and subjected to mRNA microarray analysis using Bovine Gene 1.0 ST Arrays (Affymetrix, St. Clara, CA, USA).
Contributor(s) Polei M, Koczan D, Fürbass R
Citation(s) 31952476, 32103781
Submission date Nov 13, 2018
Last update date Mar 09, 2020
Contact name Rainer Fuerbass
Organization name Leibniz Institute for Farm Animal Biology
Street address Wilhelm-Stahl-Allee
City Dummerstorf
ZIP/Postal code 18196
Country Germany
Platforms (1)
GPL16500 [BovGene-1_0-st] Bovine Gene 1.0 ST Array [transcript (gene) version]
Samples (6)
GSM3466983 primary bovine trophoblast giant cells from cow 2
GSM3466984 primary bovine uninucleate trophoblast cells from cow 2
GSM3466985 primary bovine trophoblast giant cells from cow 3
BioProject PRJNA505323

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE122474_Annotated_RMA_Matrix.xlsx 5.2 Mb (ftp)(http) XLSX
GSE122474_RAW.tar 29.2 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data are available on Series record

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