|
| Status |
Public on Dec 09, 2018 |
| Title |
RNA-Seq of PreCFU-E and CFU-E progenitors from wild type and Scf mutants |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
It has been shown previously that endothelial cells and LepR+ stromal cells are the main sources of SCF in vivo in the mouse bone marrow. We tested whether SCF from endothelial cells and/or LepR+ stromal cells is important for the maintenance of hematopoietic progenitors and erythroid progenitors in mouse bone marrow by conditional deletion of Scf from these two cell types. We discovered that Scf deletion from LepR+ stromal cells, but not endothelial cells, reduced the numbers of hematopoietic progenitors and erythroid progenitors in mice. We performed RNA-Seq on PreCFU-E and CFU-E progenitors from control mice and from mice with Scf deletion from LepR+ stromal cells. We discovered that lack of SCF from LepR+ cells induces a premature differentiation of PreCFU-E and CFU-E progenitors.
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| |
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| Overall design |
Examination of gene expression profile in 2 cell tyeps from 3 different genetic backgrounds
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| |
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| Contributor(s) |
Comazzetto S, Murphy MM, Berto S, Jeffery E, Zhao Z, Morrison SJ |
| Citation(s) |
30661958 |
| |
| Submission date |
Nov 13, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Zhiyu Zhao |
| E-mail(s) |
Zhiyu.Zhao@UTSouthwestern.edu
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Department |
Children's Medical Center Research Institute
|
| Lab |
Sean Morrison
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75390-8502 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA505314 |
| SRA |
SRP168429 |
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