|
| Status |
Public on Jul 23, 2019 |
| Title |
Gene expression profiling of developing murine Müller glia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
To study molecular changes during differentiation of retinal progenitor cells (RPCs) into Müller glia, we isolated Notch1+ cells (Notch1 is a marker of RPCs) from postnatal-day (P) 0, 3, 7, and 14 retinas,as well as Glast + cells (Glast/Slc1a3 is a marker of Müller glia precursors and Müller glia in the retina) from P7, P14, P21, and P28 retinas. We studied gene expression in these cells using MEEBO microarrays.
|
| |
|
| Overall design |
To characterize and compare P0, P3, P7, and P14 Notch1+ cells (RPCs) as well as P7, P14, P21, and P28 Glast+ cells (Müller glia precursors and adult Müller glia), RNA extracted from these cells was used for microarray analysis. A total of two-three independent biological replicates at each time point were obtained for comparative profiling of purified cells.
|
| |
|
| Contributor(s) |
Ivanov D |
| Citation(s) |
31276697 |
| |
| Submission date |
Nov 08, 2018 |
| Last update date |
Oct 22, 2019 |
| Contact name |
Dmitry Ivanov |
| E-mail(s) |
divanov@med.miami.edu
|
| Phone |
305-482-4230
|
| Organization name |
University of Miami Miller School of Medicine
|
| Department |
Ophthalmology
|
| Street address |
1638 NW 10th Avenue
|
| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL25621 |
mouse exonic evidence-based oligonucleotide (MEEBO) microarray [34288 probes] |
|
| Samples (21)
|
|
| This SubSeries is part of SuperSeries: |
| GSE122337 |
Development and epigenetic plasticity of murine Müller glia |
|
| Relations |
| BioProject |
PRJNA504551 |
Less...
More...