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Series GSE122197 Query DataSets for GSE122197
Status Public on Jul 06, 2019
Title Surface PEGylation Suppresses Pulmonary Effects of CuO in Allergen-Induced Lung Inflammation
Organism Mus musculus
Experiment type Expression profiling by array
Summary The objective of the study was to investigate how pristine CuO modulates allergic lung inflammation and whether surface modifications can influence its reactivity. CuO materials exacerbated the existing allergic airway inflammation by eliciting dramatic pulmonary neutrophilia. Transcriptomic analysis showed that CuO, CuO COOH and CuO NH3 commonly enriched neutrophil-related biological processes in lungs with and without the pre-existing condition. In contrast, CuO PEG had a significantly lower potential in triggering pulmonary changes revealing that surface PEGylation suppresses the effects triggered by the pristine material.
Overall design Pristine CuO (CuO) and its carboxylated (CuO COOH), methylaminated (CuO NH3) and PEGylated (CuO PEG) derivates were investigated in a murine asthma model. In the used model, each mouse (eight per group) received intraperitoneally a mixture of 50 mg of OVA and 2 mg of aluminum/magnesium hydroxide in 100 ml of PBS on day 1 and 10. After 10-day recovery, mice were exposed to 50 ml of PBS or 50 mg of OVA in 50 ml of PBS with or without dispersed CuO nanomaterials via oropharyngeal aspiration in isoflurane anaesthesia on four consecutive days. CuO materials were tested at three doses – 2.5, 10 and 40 mg/mouse/administration. Bronchoalveolar lavage was collected for cytological analysis and lung tissue samples for histological assessment and Agilent microarrays.
Contributor(s) Ilves M, Kinaret PA, Ndika J, Karisola P, Marwah V, Fortino V, Fedutik Y, Correia M, Ehrlich N, Loeschner K, Besinis A, Vassallo J, Handy RD, Wolff H, Savolainen K, Greco D, Alenius H
Citation(s) 31277695
To be added
Submission date Nov 05, 2018
Last update date Jul 25, 2019
Contact name Harri Alenius
Organization name University of Helsinki
Department Human Microbiome Research Program
Lab AleniusLab
Street address Haartmaninkatu 3
City Helsinki
ZIP/Postal code 00290
Country Finland
Platforms (1)
GPL21810 Agilent-074809 SurePrint G3 Mouse GE v2 8x60K Microarray [Feature Number version]
Samples (89)
GSM3460175 CuO COOH 2.5 + OVA S1
GSM3460177 CuO 2.5 S1
GSM3460179 CuO COOH 10 S1
BioProject PRJNA503901

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Supplementary file Size Download File type/resource
GSE122197_RAW.tar 532.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data provided as supplementary file
Processed data included within Sample table

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