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Series GSE122156 Query DataSets for GSE122156
Status Public on Apr 03, 2019
Title Dysfunction and stemness of tumor-infiltrating T cells are triggered by a common mechanism
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The paradox of tumor immunology is that tumor infiltrating lymphocytes (TILs) are dysfunctional in situ and yet are capable of stem cell-like behavior with self-renewal, massive expansion, multipotency, and eradication of large metastatic tumors. Here we report that the overabundance of potassium in the tumor microenvironment (TME) can explain both phenomena.
Overall design Ctrl (or Samples labelled V- Vehicle) and elevated potassium (or Samples labelled K) were used to perfrom ChiP assays. CD8+ T cells from pmel mice were stimulated in vitro with 1 μM human gp100 peptide (10 μg/ml) in Ctrl (V) or elevated potassium (K) for 5 days and secondary re-stimulation was done for 48hrs with plate-bound anti-CD3 (1 μg/ml; BD Biosciences) and soluble anti-CD28 (1 μg/ml; BD Biosciences) and expanded in culture medium (Ctrl /K) containing 60 IU/mL of IL-2 for a total of 10 days.
Contributor(s) Vodnala SK
Citation(s) 30923193
Submission date Nov 05, 2018
Last update date Apr 03, 2019
Contact name Suman Kumar Vodnala
Phone 2408583809
Organization name NCI
Department Surgery Branch
Lab Nicholas Restifo Lab
Street address 10 Center Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (2)
GPL9185 Illumina Genome Analyzer (Mus musculus)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (10)
GSM3457000 H3K9Ac_V1
GSM3457001 H3K9Ac_V2
GSM3457002 H3K9Ac_K1
BioProject PRJNA503845
SRA SRP167854

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Supplementary file Size Download File type/resource
GSE122156_27me3_common_peaks_V_K.bed.gz 213.5 Kb (ftp)(http) BED
GSE122156_H3K9Ac_common_peaks_V_K.bed.gz 320.2 Kb (ftp)(http) BED 515.1 Mb (ftp)(http) BW 397.5 Mb (ftp)(http) BW 482.4 Mb (ftp)(http) BW 433.1 Mb (ftp)(http) BW
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Raw data are available in SRA
Processed data are available on Series record

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