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Series GSE121977 Query DataSets for GSE121977
Status Public on Sep 30, 2019
Title RORa Controls Intestinal Homeostasis via Negative Regulation of NF-kB Signaling Pathway
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary A member of the nuclear receptors, retinoic acid-related orphan receptor a (RORa) is an important transcription factor for various biological processes including circadian rhythm, metabolic regulation, immune cell development and cancer. Here we found that RORa is important for intestinal homeostasis by negatively regulating the transcriptional activity of NF-kB, a major inflammatory regulator in intestinal epithelial cells. Intestinal Rora-deficient mice found were highly susceptible to DSS-induced injury. Transcriptome analysis showed that intestine-specific Rora deletion causes regulatory impairment of NF-kB signaling leading to excessive inflammatory responses. RORa specifically binds to the NF-kB target promoter and inhibits transcriptional activity for transcriptional repression of NF-kB activity via histone deacetylase 3 (HDAC3). Taken together, RORa plays a pivotal role in the homeostatic regulation of intestinal epithelial cells in inflammatory conditions. Therefore, therapeutic strategies designed to modulate RORa activity may be beneficial in the treatment of chronic inflammatory diseases such as Inflammatory bowel disease (IBD).
 
Overall design Gene expression profiling of RORaf/f and intestine-specific Rora knock-out (RORaΔIEC) Intestinal Ephithelial Cell (IEC) untreated or treated with DSS.
 
Contributor(s) Kim D, Park D, Baek S
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Submission date Oct 30, 2018
Last update date Oct 02, 2019
Contact name Dongha Kim
E-mail(s) hidongha@gmail.com
Organization name Seoul National University
Street address 599 Gwanak-ro
City Seoul
ZIP/Postal code 08826
Country South Korea
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (8)
GSM3452428 RORaf/fcontrol-rep.1
GSM3452429 RORaf/fcontrol-rep.2
GSM3452430 RORaΔIECcontrol-rep.1
Relations
BioProject PRJNA501886
SRA SRP167246

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Supplementary file Size Download File type/resource
GSE121977_RAW.tar 14.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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