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| Status |
Public on Oct 27, 2018 |
| Title |
Application of Chromatrap® Solid-State Chromatin Immunoprecipitation (ChIP) for epigenetic profiling of insects |
| Organisms |
Drosophila melanogaster; Nicrophorus vespilloides |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We report the application of Chromatraps® Solid-State Chromatin Immunoprecipitation technology for epigenetic profiling of histone modifications in insects. Here, we present the optimised protocol and conditions of Chromatrap® kits for successful ChIP and high-throughput sequencing of established model species, Drosophila melanogaster and the emerging model for behavioural plasticity Nicrophorus vespilloides. We highlight successful ChIP-seq of Drosophila melanogaster (Oregon-R) of comparable quality to modENCODE data and present successful enrichment of histone marks for Nicrophorus vespilloides. The addition of this insect-based ChIP-seq protocol provides a set of optimal guidelines to aid streamline end-users epigenetic research focus and reduce experimental time.
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| Overall design |
Optimization of ChIP-seq protocol for insect tissue of two species; Drosophila melanogaster (strain: Oregon-R) and Nicrophorus vespilloides at two different life stages; 3rd instar larvae and adult whole brains respectively. Four replicates per insect investigated using one antibody; H3K4me3 including input controls for each ChIP assay.
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| Contributor(s) |
Generalovic TN, Cunningham CB, Johnson A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Oct 26, 2018 |
| Last update date |
Feb 20, 2019 |
| Contact name |
Tomas Nicholas Generalovic |
| E-mail(s) |
793305@swansea.ac.uk
|
| Organization name |
Swansea University
|
| Department |
Bioscience
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| Lab |
Cunningham Lab
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| Street address |
Swansea University, Singleton Campus
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| City |
Swansea |
| State/province |
West Glamorgan |
| ZIP/Postal code |
SA2 8PP |
| Country |
United Kingdom |
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| Platforms (2) |
| GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
| GPL25731 |
Illumina MiSeq (Nicrophorus vespilloides) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA498662 |
| SRA |
SRP166955 |
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