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| Status |
Public on Apr 10, 2019 |
| Title |
Loss of DUX causes minor defects in zygotic genome activation and is compatible with mouse development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
How maternal factors in oocytes trigger zygotic genome activation (ZGA) is a long-standing question in developmental biology. Recent studies in 2-cell like embryonic stem cells (2C-like cells) implicate that the DUX family transcription factors are key regulators of ZGA in placental mammals. To characterize the role of DUX in ZGA, we generated Dux cluster knockout (KO) mouse lines. Unexpectedly, we found both Dux zygotic KO (Z-KO) and maternal/zygotic KO (MZ-KO) embryos can survive to adulthood despite showing reduced developmental potential. Furthermore, transcriptome profiling of the MZ-KO embryos revealed that loss of DUX has minimal effect on ZGA and most DUX targets in 2C-like cells are normally activated in MZ-KO embryos. Thus, contrary to the key function in inducing 2C-like cells, our data indicate that DUX only has a minor role in ZGA and loss of DUX is compatible with mouse development.
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| Overall design |
To assess the role of Dux in mouse zygotic genome activation, RNA sequencing (RNA-seq) were performed for Dux wildtype (WT) and maternal-zygotic knockout (MZ-KO) 1-cell and 2-cell embryos
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| Contributor(s) |
Chen Z, Zhang Y |
| Citation(s) |
31133747 |
| |
| Submission date |
Oct 24, 2018 |
| Last update date |
Jul 10, 2019 |
| Contact name |
Zhiyuan Chen |
| E-mail(s) |
Zhiyuan.chen@cchmc.org
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| Organization name |
Cincinnati Children's Hospital Medical Center
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| Street address |
3333 Burnet Avenue
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| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA498305 |
| SRA |
SRP166778 |
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