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Series GSE121684 Query DataSets for GSE121684
Status Public on Feb 05, 2019
Title Identification of RNAs bound to PUM2 in Norad+/+ and Norad-/- brains [CLIP-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Although numerous long noncoding RNAs (lncRNAs) have been identified, our understanding of their roles in mammalian physiology remains limited. Here we investigated the physiologic function of the conserved lncRNA Norad in vivo. Deletion of Norad in mice results in genomic instability and mitochondrial dysfunction, leading to a dramatic multi-system degenerative phenotype resembling premature aging. Loss of tissue homeostasis in Norad-deficient animals is attributable to augmented activity of PUMILIO proteins, which act as post-transcriptional repressors of target mRNAs to which they bind. Norad is the preferred RNA target of PUMILIO2 (PUM2) in mouse tissues and, upon loss of Norad, PUM2 hyperactively represses key genes required for mitosis and mitochondrial function. Accordingly, enforced Pum2 expression fully phenocopies Norad deletion, resulting in rapid-onset aging-associated phenotypes. These findings provide new insights and open new lines of investigation into the roles of noncoding RNAs and RNA binding proteins in normal physiology and aging.
Overall design RNAs bound to PUM2 were identified using enhanced UV crosslinking immunoprecipitation with high-throughput sequencing (eCLIP).
Contributor(s) Kopp F, Chen B, Zhang H, Lee S, Xie Y, Mendell JT
Citation(s) 30735131
Submission date Oct 23, 2018
Last update date Mar 22, 2019
Contact name Joshua Mendell
Organization name UT Southwestern Medical Center
Street address 6000 Harry Hines Blvd., NA6.200A
City Dallas
State/province TX
ZIP/Postal code 75390-9148
Country USA
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (8)
GSM3443313 BRN_WT_input_A
GSM3443314 BRN_WT_input_B
GSM3443315 BRN_WT_IP_A
BioProject PRJNA498094
SRA SRP166550

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE121684_PUM2_CLIP_clusters_KO.txt.gz 315.2 Kb (ftp)(http) TXT
GSE121684_PUM2_CLIP_clusters_WT.txt.gz 235.4 Kb (ftp)(http) TXT
Raw data are available in SRA
Processed data are available on Series record

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