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| Status |
Public on Apr 29, 2019 |
| Title |
Requirements for multivalent Yb body assembly in transposon silencing in Drosophila |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Female sterile (1) Yb (Yb) is a primary component of Yb bodies, perinuclear foci known as the site of PIWI-interacting RNA (piRNA) biogenesis in Drosophila ovarian somatic cells. Yb consists of Helicase C-terminal (Hel-C), RNA helicase and extended Tudor (eTud) domains. We previously showed that the RNA helicase domain is necessary for Yb−RNA interaction, Yb body formation and piRNA biogenesis. Here, we investigated the functions of the two other domains and found that Hel-C and eTud are necessary for Yb to self-associate and to interact with RNAs and other Yb body components, respectively. Both domains were essential for Yb body formation and transposon silencing. Without eTud, piRNA production was completely impaired. Loss of Hel-C severely reduced the levels of transposon-targeting piRNAs, although genic piRNAs unrelated to transposon silencing were still produced. Similar phenotypes were observed when mutations in flamenco, the primary source of transposon-targeting piRNAs, led to the expression of attenuated RNAs. Yb bodies are liquid-like multivalent condensates whose assembly depends on Yb self-association and widespread Yb−flamenco RNA binding.
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| Overall design |
Four piRNA-seq libraries: piRNA libraries were prepared from RNA associated with Flag-Piwi immunoprecipitates (with size selection at 23-30 nt).
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| Contributor(s) |
Hirakata S, Siomi MC |
| Citation(s) |
31267711 |
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| Submission date |
Oct 22, 2018 |
| Last update date |
Jul 21, 2019 |
| Contact name |
Shigeki Hirakata |
| Organization name |
The University of Tokyo
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| Department |
Department of Biological Sciences
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| Lab |
Siomi Lab
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| Street address |
2-11-16 Yayoi, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
113-0032 |
| Country |
Japan |
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| Platforms (1) |
| GPL16479 |
Illumina MiSeq (Drosophila melanogaster) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA497868 |
| SRA |
SRP166327 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE121605_processed_data.txt.gz |
90.1 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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