|
| Status |
Public on Jan 31, 2019 |
| Title |
Genomic binding profile of Junctophilin 2 N-terminal truncate (JP2NT) and the effect of JP2NT overexpreesion on cardiac transcriptional reprogramming in response to Transverse Aortic Banding |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Calpain-mediated cleavage transfroms the full-length JP2 from a structural protein into a transcriptional regulator (JP2NT). We used RNA-seq to reveal the effect of JP2NT overexpression on cardiac transcriptional reprogramming induced by left ventricular pressure overload using transverse aortic banding in mice. We used ChIP-seq to reveal the genomic binding pattern of JP2NT. Additional ChIP-seq analyses against TBP and MEF2C were performed to reveal whether JP2NT overexpression affects endogeneous binding of TBP and MEF2C.
|
| |
|
| Overall design |
Cardiac specific overexpression of HA-tagged JP2NT transgene was achieved by an a-MHC-promoter driven transgenic system. The JP2NT-OE and littermate controls were subjected to transverse aortic banding (TAB) for 3 weeks. Left ventricle RNA was subjected to RNA-seq. Baseline JP2NT-OE or control hearts were subjected to ChIP-seq using antibodies against HA tag, MEF2C or TBP.
|
| |
|
| Contributor(s) |
Guo A, Wang Y, Song L |
| Citation(s) |
30409805 |
| |
| Submission date |
Oct 21, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Long-Sheng Song |
| E-mail(s) |
long-sheng-song@uiowa.edu
|
| Phone |
(319) 384-2890
|
| Organization name |
University of Iowa
|
| Department |
Department of Internal Medicine
|
| Lab |
Lab of Long-Sheng Song
|
| Street address |
285 Newton Road
|
| City |
Iowa City |
| State/province |
Iowa |
| ZIP/Postal code |
52240 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (19)
|
|
| This SubSeries is part of SuperSeries: |
| GSE121547 |
E-C coupling structural protein junctophilin-2 encodes a stress-adaptive transcription regulator |
|
| Relations |
| BioProject |
PRJNA497730 |
| SRA |
SRP166231 |
Less...
More...