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Series GSE121319 Query DataSets for GSE121319
Status Public on Jul 16, 2019
Title GPRC5A facilitates cell proliferation and bone metastasis of prostate cancer.
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Prostate cancer is a high frequent disease. Early stage prostate cancer can be cured with high probability by early treatment, but hormone refractory prostate cancer and progressive prostate cancer is poor prognosis. Orphan GPCRs have the potential to become a drug discovery target in various carcinomas, but there are few reports in prostate cancer and there are still many possibilities. By big data analysis, we focused on the biological properties of the prostate cancer cell line and found GPRC5A from Orphan GPCR as one of the regulators of prostate cancer development. GPRC5A knock out by genomic editing in PC3, proliferative ability and bone metastasis was markedly suppressed. In prostate cancer cells, GPRC5A inhibit phosphorylation of CREB and affected the expression of cell cycle related genes. GPRC5A is also involved in the establishment of bone metastases, and correlated with prostate cancer metastasis and prognosis. These dates suggest that GPRC5A facilitates cell proliferation and progression of prostate cancer and can be a target for new prognostic marker or drug discovery of prostate cancer.
Overall design mRNA profiles of wild type PC3-Luc cells and two GPRC5A knockout PC3-Luc cells were generated by deep sequencing, in triplicate, using Illumina Miseq.
Contributor(s) Imai Y, Sawada Y
Citation(s) 31276604
Submission date Oct 16, 2018
Last update date Jul 16, 2019
Contact name Yuuki Imai
Phone +81-89-960-5925
Organization name Ehime University
Department Proteo-Science Center
Lab Division of Integrative Pathophysiology
Street address Shitsukawa
City Toon
State/province Ehime
ZIP/Postal code 791-0295
Country Japan
Platforms (1)
GPL15520 Illumina MiSeq (Homo sapiens)
Samples (9)
GSM3431438 PC3_Control_rep1
GSM3431439 PC3_Control_rep2
GSM3431440 PC3_Control_rep3
BioProject PRJNA497056
SRA SRP165862

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Supplementary file Size Download File type/resource
GSE121319_tmm_PC3_Control_vs_GPRC5AKO.txt.gz 3.4 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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