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| Status |
Public on Jan 09, 2019 |
| Title |
ChIP-seq analysis of androgen receptor binding in epididymis of ArKI mice |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
To analyze how SUMOylation of AR affects its chromatin binding, we generated a mouse model (ArKI) in which the two conserved SUMO acceptor lysines of androgen receptor (AR) were permanently abolished by converting them to arginines (ArK381R, K500R). ChIP-seq was used to analyse how the lack of AR SUMOylation affects the chromatin binding of AR in epididymis.
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| Overall design |
For each sample, caput epididymides (containing initial segments) from three wild type (WT) or ArKI (KI) mice were combined, chromatin binding of AR was analyzed using ChIP and sequenced using Illumina HiSeq 3000 system. We generated two replicates from WT and ArKI mice.
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| Contributor(s) |
Niskanen EA, Palvimo JJ, Malinen M, Sipilä P |
| Citation(s) |
30770815 |
| |
| Submission date |
Oct 11, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Einari A Niskanen |
| E-mail(s) |
einari.niskanen@uef.fi
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| Organization name |
University of Eastern Finland
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| Department |
School of Medicine
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| Lab |
Biomedicine
|
| Street address |
Yliopistonranta 8
|
| City |
Kuopio |
| ZIP/Postal code |
70210 |
| Country |
Finland |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE121152 |
RNA-seq and ChIP-seq analyses of epididymis from ArKI mice |
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| Relations |
| BioProject |
PRJNA495852 |
| SRA |
SRP165231 |
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