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| Status |
Public on Oct 05, 2021 |
| Title |
Correlation of clinical parameters of heart failure with myocardial gene expression in dilated cardiomyopathy |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To gain new insights into the complex pathophysiology of dilated cardiomyopathy (DCM) we performed a quantitative approach to identify genes with expression patterns that linearly correlate with parameters of cardiac morphology (left ventricular end-diastolic diameter indexed by body surface are (LVEDDI), systolic function (LV ejection fraction (LVEF)), and serum levels of cardiac peptide hormone N-terminal pro-brain natriuretic peptide (NT-proBNP) in human endomyocardial biopsies of 47 DCM patients and 8 individuals with normal LVEF. A set of genes was identified as common heart failure markers characterized by correlation of their expression with cardiac morphology, systolic function and NT-proBNP. Among them are already known genes encoding e.g. the natriuretic peptide hormones NPPA and NPPB and its converting enzyme corin, but also potential new HF markers like EP300 antisense RNA1 and dimethylarginine dimethylaminohydrolase 1 (DDAH1) along with other genes with so far unknown relation to heart function. In contrast, the expression of other genes including the Ca2+ flux regulating genes phospholamban (PLN), sarcoplasmic/endoplasmic reticulum calcium ATPase 2 (SERCA), and extracellular matrix proteins showed significant correlation with LVEF and LVEDDI only. Those genes seem to reflect more specifically pathological alterations of systolic function and morphology in DCM hearts.
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| Overall design |
This study comprises 47 DCM patients with LV systolic dysfunction (LVEF <45%) and symptoms of HF according to the New York Heart Association (NYHA) functional class II and III . Data from a subset of these patients had been reported in regard to their response on an intervention previously (Ameling et al, 2013). Transcriptome analyses were performed from endomyocardial biopsies which were no longer needed for routine diagnostics. Written informed consent was obtained by each patient and the protocol was approved by the Ethics Committee of the University of Greifswald, Germany and to the principles of the Declaration of Helsinki.
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| Contributor(s) |
Witt E, Hammer E, Dörr M, Weitmann K, Beug D, Nauck M, Völker U, Felix SB, Ameling S |
| Citation(s) |
31274368 |
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| Submission date |
Oct 05, 2018 |
| Last update date |
Nov 29, 2021 |
| Contact name |
Sabine Ameling |
| Organization name |
Universitätsmedizin Greifswald
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| Department |
Interfakultäres Institut für Genetik und Funktionelle Genomforschung
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| Lab |
Funktionelle Genomforschung
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| Street address |
Jahnstraße 15a
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| City |
Greifswald |
| ZIP/Postal code |
17487 |
| Country |
Germany |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (55)
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| Relations |
| BioProject |
PRJNA494858 |
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