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Series GSE120661 Query DataSets for GSE120661
Status Public on Jan 31, 2019
Title Identification of Biomarkers of Vaccine Reactogenicity
Organism Mus musculus
Experiment type Expression profiling by array
Summary Systems vaccinology approaches have been used to successfully define early signatures of the vaccine-induced immune response. However, the possibility that transcriptomics can also predict vaccine reactogenicity has not been fully explored. We have compared four licensed vaccines that have a demonstrated safety profile, as well as three agonists of TLRs that have a known inflammatory potential, to elucidate the transcriptomic profile of an acceptable response to a vaccination versus those which push the inflammatory envelope. In mice, we looked at the transcriptomic changes in the injected muscle, the lymph node that drained the muscle and the PBMC isolated from the circulating blood from the very early timepoint of 4 hours to over the period of one week. A detailed examination and comparative analysis of the transcriptome from each of these tissues, at all the timepoints of 4, 8, 24, 48, 72 and 168 hr and with these eight different vaccines, TLR or saline control injections, revealed a set of novel biomarkers that are reflective of inflammation after vaccination. These biomarkers are easily sampled and readily measurable in the peripheral blood, providing a useful tool to predict levels of reactogenicity, as a way to select candidates with acceptable immunogenicity and safety profiles.
Overall design High-quality RNA samples isolated from muscle tissue excised around the injection site, the draining medial iliac lymph nodes (MLN) and total PBMCs of mice at 4, 8, 24, 48, 72, 168 hours after the vaccination were subjected to genome-wide transcriptome analysis.
Contributor(s) McKay PF, Cizmeci D
Citation(s) 31084714
Submission date Sep 29, 2018
Last update date Jun 20, 2019
Contact name Deniz Cizmeci
Organization name Imperial College London
Street address Imperial College London
City London
ZIP/Postal code W2 1NY
Country United Kingdom
Platforms (1)
GPL10787 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version)
Samples (839)
GSM3407136 ENG_BL_0h_1
GSM3407137 ENG_BL_0h_2
GSM3407138 ENG_BL_0h_3
BioProject PRJNA494386

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE120661_RAW.tar 9.7 Gb (http)(custom) TAR (of TXT)
Raw data provided as supplementary file
Processed data included within Sample table

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