 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 19, 2018 |
| Title |
Single cell transcriptome profiling of mouse and hESC-derived pancreatic progenitors |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Human embryonic stem cells (hESCs) are a potential unlimited source of insulin-producing β-cells for diabetes treatment. A greater understanding of how β-cells form during embryonic development will improve current hESC differentiation protocols. As β-cells are formed from NEUROG3-expressing endocrine progenitors, this study focused on characterizing the single-cell transcriptomes of mouse and hESC-derived endocrine progenitors. To do this, 7,223 E15.5 and 6,852 E18.5 single cells were isolated from Neurog3-Cre; Rosa26mT/mG embryos, allowing for enrichment of endocrine progenitors (yellow; tdTomato + EGFP) and endocrine cells (green; EGFP). From a NEUROG3-2A-eGFP CyT49 hESC reporter line (N5-5), 4,497 hESC-derived endocrine progenitor cells were sequenced. Differential expression analysis reveals enrichment of markers that are consistent with progenitor, endocrine, or novel cell-state populations. This study characterizes the single-cell transcriptomes of mouse and hESC-derived endocrine progenitors and serves as a resource (https://lynnlab.shinyapps.io/embryonic_pancreas/) for improving the formation of functional β-like cells from hESCs.
|
| |
|
| Overall design |
This study characterizes the single-cell transcriptomes of mouse and hESC-derived endocrine progenitors
|
| |
|
| Contributor(s) |
Krentz NA, Lee M, Xu EE, Sasaki S, Maslova S, Sproul S, Lynn FC |
| Citation(s) |
30540962 |
| |
| Submission date |
Sep 26, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Francis C Lynn |
| E-mail(s) |
fclynn@interchange.ubc.ca
|
| Phone |
6048752000
|
| Organization name |
University of British Columbia
|
| Department |
Surgery
|
| Lab |
Lynn
|
| Street address |
950 28th Ave W
|
| City |
Vancouver |
| State/province |
BC |
| ZIP/Postal code |
V5Z 4H4 |
| Country |
Canada |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA493263 |
| SRA |
SRP162688 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE120522_E15_aggregation_files.tar.gz |
99.1 Mb |
(ftp)(http) |
TAR |
| GSE120522_E18_aggregation_files.tar.gz |
89.3 Mb |
(ftp)(http) |
TAR |
| GSE120522_E18_endocrine_aggregation_files.tar.gz |
52.0 Mb |
(ftp)(http) |
TAR |
| GSE120522_GRCh38_genes.gtf.gz |
40.2 Mb |
(ftp)(http) |
GTF |
| GSE120522_GRCh38_genome.fa.gz |
840.8 Mb |
(ftp)(http) |
FA |
| GSE120522_RAW.tar |
254.2 Mb |
(http)(custom) |
TAR (of MTX, TSV, TXT) |
| GSE120522_README_aggregation_datasets.txt |
2.8 Kb |
(ftp)(http) |
TXT |
| GSE120522_mm10_genes.gtf.gz |
25.4 Mb |
(ftp)(http) |
GTF |
| GSE120522_mm10_genome.fa.gz |
769.1 Mb |
(ftp)(http) |
FA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...