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| Status |
Public on Aug 16, 2008 |
| Title |
ARGONAUTE-small RNA Interactions in Arabidopsis thaliana Identified by High-Throughput Sequencing |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The goal of this study was to identify small RNA-ARGONAUTE interactions in Arabidopsis. Co-immunoprecipitation assays followed by high-throughput sequencing were done to identify small RNA associated with HA-AGO2 and HA-AGO7.
Keywords: small RNA - ARGONAUTE interactions
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| Overall design |
Sequencing-by-synthesis technology was used to sequence small RNA from input and co-immunoprecipitation fractions from cell lysates of inflorescence tissue from Arabidopsis plants transformed with HA epitope-tagged AGO2 or AGO7 constructs. Meaningful intepretation requires comparison between the input and co-immunoprecipitation datasets.
Fastq files are not available for this series as only ELAND output files were provided by the sequencing facility at the time of sequencing (2007).
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| Contributor(s) |
Montgomery TA |
| Citation(s) |
18342362 |
| |
| Submission date |
Jul 08, 2008 |
| Last update date |
Oct 13, 2015 |
| Contact name |
James C Carrington |
| E-mail(s) |
jcarrington@danforthcenter.org
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| Phone |
314-587-1202
|
| Organization name |
Donald Danforth Plant Science Center
|
| Lab |
James C. Carrington
|
| Street address |
975 North Warson Road
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
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| Platforms (1) |
| GPL7029 |
Carrington-Arabidopsis-Illumina-miRNA-AGO-high-throughput sequencing |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA113357 |
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