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Series GSE12037 Query DataSets for GSE12037
Status Public on Aug 16, 2008
Title ARGONAUTE-small RNA Interactions in Arabidopsis thaliana Identified by High-Throughput Sequencing
Organism Arabidopsis thaliana
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary The goal of this study was to identify small RNA-ARGONAUTE interactions in Arabidopsis. Co-immunoprecipitation assays followed by high-throughput sequencing were done to identify small RNA associated with HA-AGO2 and HA-AGO7.

Keywords: small RNA - ARGONAUTE interactions
 
Overall design Sequencing-by-synthesis technology was used to sequence small RNA from input and co-immunoprecipitation fractions from cell lysates of inflorescence tissue from Arabidopsis plants transformed with HA epitope-tagged AGO2 or AGO7 constructs. Meaningful intepretation requires comparison between the input and co-immunoprecipitation datasets.

Fastq files are not available for this series as only ELAND output files were provided by the sequencing facility at the time of sequencing (2007).
 
Contributor(s) Montgomery TA
Citation(s) 18342362
Submission date Jul 08, 2008
Last update date Oct 13, 2015
Contact name James C Carrington
E-mail jcarrington@danforthcenter.org
Phone 314-587-1202
Organization name Donald Danforth Plant Science Center
Lab James C. Carrington
Street address 975 North Warson Road
City Saint Louis
State/province MO
ZIP/Postal code 63132
Country USA
 
Platforms (1)
GPL7029 Carrington-Arabidopsis-Illumina-miRNA-AGO-high-throughput sequencing
Samples (4)
GSM304282 HA-AGO2 Input Fraction Small RNA
GSM304283 HA-AGO2 Co-IP Fraction Small RNA
GSM304284 HA-AGO7 Input Fraction Small RNA
Relations
BioProject PRJNA113357

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE12037_RAW.tar 276.5 Mb (http)(custom) TAR (of TXT)
Raw data not provided for this record
Processed data included within Sample table
Processed data provided as supplementary file

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