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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 18, 2019 |
| Title |
Transcriptomic signatures indicative of a non-genotoxic adverse outcome pathway for small intestine cancer in the duodenum of mice exposed to hexavalent chromium, captan, or folpet |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We evaluated duodenal tissues of B6C3F1 mice exposed to either Cr(VI) (180ppm in drinking water), captan (≤12000ppm in diet), or folpet (≤16000ppm in diet) for 28 days. TempO-Seq technology was used to measure the sentinel geneset, S1500+, representing ~3000 toxicology-relevant genes. Global exposure-induced transcriptional responses were similar between agents (all Pearson correlation coefficients ≥0.61). Focusing on responses at doses of captan and folpet that correlate most closely with effects of Cr(VI), a gene-level comparison identified that 126/546 (23%) differentially expressed genes were altered in the same direction across all three agents. Pathway-level comparisons identified 25 up-regulated pathways commonly modulated between Cr(VI), captan, and folpet. Commonly altered genes and pathways were related to cellular metabolism, stress, immune response, and cell proliferation. No up-regulated pathways relevant to genotoxicity were associated with responses to any agent.
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| Overall design |
Examination of ~3000 genes by targeted sequencing in duodenal tissue from mice from 6 treatment groups.
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| Contributor(s) |
Chappell GA, Rager JE, Thompson CM |
| Citation(s) |
31558096 |
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| Submission date |
Sep 18, 2018 |
| Last update date |
Dec 09, 2019 |
| Contact name |
Grace Chappell |
| E-mail(s) |
gchappell@toxstrategies.com
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| Organization name |
ToxStrategies, Inc.
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| Department |
Health Sciences
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| Street address |
9390 Research Blvd, Bldg 2, Ste. #100
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| City |
Austin |
| State/province |
TX |
| ZIP/Postal code |
78723 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (40)
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| Relations |
| BioProject |
PRJNA491726 |
| SRA |
SRP162050 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE120146_SP0071_gene_counts_GEO.csv.gz |
188.2 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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