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Status |
Public on Sep 13, 2018 |
Title |
Transcriptome differences between primary brown adipocytes of wildtype and UCP1-KO mice |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We analyzed coding transcript abundance in fully differentiated, primary brown adipocytes from murine, interscapular brown adipose tissue of wildtype and UCP1-KO mice.
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Overall design |
We prepared primary cells from the stromal-vascular fraction of murine interscapular brown fat and differentiated these in vitro into brown adipocytes. Three independent cultures each were generated from wildtype 129SvEvS1 mice and UCP1-KO mice on the same strain background. We sequenced total RNA and determined transcript abundance.
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Contributor(s) |
Li Y, Schweizer S, Fromme T, Klingenspor M |
Citation(s) |
30456392 |
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Submission date |
Sep 12, 2018 |
Last update date |
Mar 21, 2019 |
Contact name |
Tobias Fromme |
E-mail(s) |
fromme@tum.de
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Organization name |
Technical University of Munich
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Department |
Molecular Nutritional Medicine
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Street address |
Gregor-Mendel-Str. 2
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City |
Freising |
ZIP/Postal code |
85354 |
Country |
Germany |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (6)
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Relations |
BioProject |
PRJNA490535 |
SRA |
SRP161606 |