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Series GSE119863 Query DataSets for GSE119863
Status Public on Sep 13, 2018
Title BCL6 confers KRAS-mutant NSCLCs resistance to BET inhibitors [ChIP-Seq]
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report that BCL6 interacts with BRD3 and that BRD3 inhibition upregulates BCL6. By obtaining sequence from chromatin immunoprecipitated DNA of A549 cells, we generated genome-wide binding gene spectrums of BCL6 and BRD3. By locating -3000 to +200 bps around TSS, we find that BRD3 binds to the promoters of 2470 genes and that BCL6 binds to the promoters of 694 genes, with 82 genes shared by BRD3 and BCL6. Interestingly, BCL6 itself is one of these 82 shared genes, implying that BCL6 mediated self-repression may require the participation of BRD3. Other shared genes includes SGK2, IDH2, HOXB6, SIRT1, SOCS3 and ZFNs, demonstrating the widely involvement of BRD3 in the regulatory role of BCL6. To examine the dependency of BCL6 on BRD3, we also compared BCL6-binding spectrums with or without a BET inhibitor, OTX015. The ChIP-seq data with BCL6 antibody exhibited a diverse BCL-binding spectrum composed of 731 genes in OTX015 treatment, with only 117 genes overlapped with that of DMSO treatment. These data demonstrates that BET inhibition is sufficient to change BCL6-binding spectrum. This study provides a new insight of BCL6 regulatory roles in KRAS-mutant NSCLCs, where BRD3 is recruited by BCL6 and affect BCL6 binding preference as a cofactor.
 
Overall design Examination of BCL6 and BRD3 binding spectrums in unstressed conditions. 2. Comparation of BCL6-binding genes with or without a BET inhibitor, OTX015 (1 μM for 6 hr), by ChIP-seq assay.
 
Contributor(s) Guo J, Zou B
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Submission date Sep 12, 2018
Last update date Mar 27, 2019
Contact name Bin Zou
E-mail(s) zoubin@gzzoc.com
Organization name Sun Yat-sen University
Department Zhongshan Ophthalmic Center,
Lab State Key Laboratory of Ophthalmology
Street address Jinsui Lu 7
City Guangzhou
State/province Guangdong
ZIP/Postal code 510000
Country China
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (7)
GSM3386104 DMSO_anti-BCL6
GSM3386105 OTX015_anti-BCL6
GSM3386106 DMSO_anti-BRD3
Relations
BioProject PRJNA490505
SRA SRP161595

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119863_BCL6_DMSO_IgG_peaks.wig.gz 230.2 Kb (ftp)(http) WIG
GSE119863_BCL6_OTX015_IgG_peaks.wig.gz 276.3 Kb (ftp)(http) WIG
GSE119863_BRD3_DMSO_IgG_peaks.wig.gz 293.3 Kb (ftp)(http) WIG
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Raw data are available in SRA
Processed data are available on Series record

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