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| Status |
Public on Sep 18, 2008 |
| Title |
Segmentation-based detection of allelic imbalance and LOH in cancer cells using whole genome SNP arrays |
| Organism |
Homo sapiens |
| Experiment type |
Genome variation profiling by SNP array
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| Summary |
High-resolution microarray-based whole genome genotyping (WGG) techniques based on SNP analysis have successfully been applied in cancer genomics to study gene copy number alterations and allele-specific aberrations such as loss-of-heterozygosity (LOH). Problems in data interpretation arise when WGG is applied on tumor tissue specimens, in which normal cell components and tumor subpopulations frequently exist. Such heterogeneity may lead to reduced detection of cancer cell specific genomic alterations.
To circumvent problems with sample heterogeneity, we propose using a segmentation strategy derived from DNA copy number analysis for detection of LOH and allelic imbalance. We generated an experimental dilution series of a tumor cell line mixed with its paired normal cell line and simulated data for such dilutions to test the strategy. We also used data sets generated on both Affymetrix and Illumina WGG platforms, including paired tumor-normal samples and tumors previously characterized by FISH. We tested the segmentation strategy against several reported algorithms. We demonstrate high sensitivity and specificity of the segmentation strategy for detecting both minute and gross allelic imbalances originating from DNA copy number gain, loss, and neutral events in tumor specimens. For example, hemizygous copy number loss can be detected in samples containing only 20-25% tumor cells. Furthermore, the strategy can identify cell subpopulation specific events and accurately estimate the fraction of cells affected by an allelic imbalance.
Thus, the segmentation strategy extends the usefulness of WGG platforms for
investigation of allelic imbalances in heterogeneous tumor genomes.
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| Overall design |
The proposed segmentation strategy detects allelic imbalance originating from DNA copy number gain, loss or copy neutral events. Here we use Illumina 317K, 370K and 550K and Affymetrix 250K whole-genome single-nucleotide polymorphism arrays hybridized with tumor samples (breast, colon, leukemia, and urothelial) to evaluate the segmentation strategy against several other reported methods. The data sets include a simulated dilution series, an experimental dilution series of a breast cancer cell line as well as paired tumor-normal samples. This GEO series include the 12 sample experimental dilution series of the HCC1395 breast cancer cell line (CRL-2324: www.atcc.org) and 4 paired urothelial tumor-normal samples, all hybridized on Illumina HumanCNV 370k BeadChips.
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| Web link |
http://baseplugins.thep.lu.se/wiki/se.lu.onk.BAFsegmentation
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| Contributor(s) |
Staaf J, Lindgren D, Vallon-Christersson J, Isaksson A, Göransson H, Juliusson G, Rosenquist R, Höglund M, Borg A, Ringner M |
| Citation(s) |
18796136 |
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| Submission date |
Jul 03, 2008 |
| Last update date |
Jan 03, 2013 |
| Contact name |
Johan Staaf |
| Organization name |
SCIBLU - Swegene Centre for Integrative Biology at Lund University
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| Street address |
Medicon Village
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| City |
Lund |
| ZIP/Postal code |
SE-223 81 |
| Country |
Sweden |
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| Platforms (1) |
| GPL6986 |
Illumina HumanCNV370-Duov1 DNA Analysis BeadChip (HumanCNV370v1) |
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| Samples (20)
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| GSM302883 |
Lymphoblastoid cell line HCC1395BL (CRL2325) |
| GSM302884 |
Breast carcinoma cell line HCC1395 diluted with HCC1395BL (CRL2324_23pc) |
| GSM302885 |
Breast carcinoma cell line HCC1395 diluted with HCC1395BL (CRL2324_47pc) |
| GSM302886 |
Breast carcinoma cell line HCC1395 (CRL2324) |
| GSM302887 |
Breast carcinoma cell line HCC1395 diluted with HCC1395BL (CRL2324_79pc) |
| GSM302888 |
Breast carcinoma cell line HCC1395 diluted with HCC1395BL (CRL2324_50pc) |
| GSM302889 |
Breast carcinoma cell line HCC1395 diluted with HCC1395BL (CRL2324_45pc) |
| GSM302890 |
Breast carcinoma cell line HCC1395 diluted with HCC1395BL (CRL2324_34pc) |
| GSM302891 |
Breast carcinoma cell line HCC1395 diluted with HCC1395BL (CRL2324_30pc) |
| GSM302892 |
Breast carcinoma cell line HCC1395 diluted with HCC1395BL (CRL2324_21pc) |
| GSM302893 |
Breast carcinoma cell line HCC1395 diluted with HCC1395BL (CRL2324_14pc) |
| GSM302894 |
Breast carcinoma cell line HCC1395 diluted with HCC1395BL (CRL2324_10pc) |
| GSM302906 |
Urothelial carcinoma (UC152_I) |
| GSM302907 |
Urothelial carcinoma (UC288_R) |
| GSM302908 |
Urothelial carcinoma (UC513_M1) |
| GSM302909 |
Urothelial carcinoma (UC395_I_M2) |
| GSM302910 |
Blood (UC152_B) |
| GSM302911 |
Blood (UC288_B) |
| GSM302912 |
Blood (UC395_B) |
| GSM302913 |
Blood (UC513_B) |
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| Relations |
| BioProject |
PRJNA105853 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11976_GEO_Export_Urothelial_INT_370k.txt |
89.9 Mb |
(ftp)(http) |
TXT |
| GSE11976_GEO_export_DilutionSeries_INT_370k.txt |
132.0 Mb |
(ftp)(http) |
TXT |
| GSE11976_RAW.tar |
38.9 Mb |
(http)(custom) |
TAR |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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