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Series GSE119697 Query DataSets for GSE119697
Status Public on Jan 31, 2019
Title Exploration of CTCF post-translation modifications uncovers Serine-224 phosphorylation by PLK1 at pericentric regions during the G2/M transition
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Other
Summary The zinc finger CCCTC-binding protein (CTCF) carries out many, at times contradictory, functions in the cell. Although previous studies have sought to explain CTCF multivalency based on sequence composition of binding sites, few have examined how CTCF post-translational modification (PTM) could contribute to the its many functions. Here, we performed CTCF mass spectrometry, identified a novel phosphorylation site at Serine 224 (Ser224-P), and demonstrate that phosphorylation is carried out by Polo kinase 1 (PLK1). CTCF Ser224-P is chromatin-associated, mapping to at least a subset of known CTCF sites. Interestingly, CTCF Ser224-P accumulates during the G2/M transition of the cell cycle and is enriched at pericentric regions. To test function, we mutated residue 224. The phospho-obviation mutant, S224A, appeared normal. However, the phospho-mimic mutant, S224E, is detrimental to mouse embryonic stem cell colonies. While ploidy and chromatin architecture appear unaffected, S224E mutants exhibit differential expression of hundreds of genes, including p53 and p21. We have thus identified a new CTCF PTM and provided evidence of function during cellular growth and gene regulation.
 
Overall design CTCF and CTCF Ser224-P ChIP-seq, RNA-seq, Hi-C^2
 
Contributor(s) Del Rosario BC, Kriz AJ, Del Rosario AM, Anselmo A, Fry CJ, White FM, Sadreyev RI, Lee JT
Citation(s) 30676316
Submission date Sep 09, 2018
Last update date May 10, 2019
Contact name Andrea June Kriz
E-mail(s) ajkriz@g.harvard.edu
Phone 6177265943
Organization name Massachusetts General Hospital, Harvard Medical School
Department Molecular Biology
Lab Jeannie Lee Lab
Street address 185 Cambridge St
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (13)
GSM3380965 CTCF input DNA, rep1
GSM3380966 CTCF ChIP DNA, rep1
GSM3380967 CTCF Ser224-P ChIP DNA, rep1
Relations
BioProject PRJNA490032
SRA SRP160448

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119697_RAW.tar 1.9 Gb (http)(custom) TAR (of BW, HIC)
GSE119697_RNA_S224E_counts.rep1.txt.gz 1.1 Mb (ftp)(http) TXT
GSE119697_RNA_S224E_counts.rep2.txt.gz 1.1 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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