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Series GSE119582 Query DataSets for GSE119582
Status Public on Sep 01, 2019
Title Transcriptional profile of Mycobacterium tuberculosis mycP1-eccE1 deletion mutant
Organism Mycobacterium tuberculosis
Experiment type Expression profiling by high throughput sequencing
Summary Mycobacterium tuberculosis is a slow-growing intracellular bacterium with the ability to induce host cell death and persist indefinitely in the human body. This pathogen uses the specialised ESX-1 secretion system to secrete virulence factors and potent immunogenic effectors required for disease progression. ESX-1 is a multi-subunit apparatus with a membrane complex that is predicted to form a pore in the cytoplasmic membrane. In M. tuberculosis this complex is made of five membrane proteins: EccB1, EccCa1, EccCb1, EccD1, EccE1. In this study, we have characterised the membrane component EccE1, and we found that deletion of eccE1 leads to disruption of ESX-1 secretion and attenuation of M. tuberculosis ex vivo.
 
Overall design In this study, we have characterized the membrane component EccE1, and we found that deletion of eccE1 leads to disruption of ESX-1 secretion and attenuation of M. tuberculosis ex vivo.
 
Contributor(s) Benjak A, Soler P, Cole ST
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Submission date Sep 06, 2018
Last update date Sep 01, 2019
Contact name Andrej Benjak
E-mail(s) andrej.benjak@epfl.ch
Organization name École Polytechnique Fédérale de Lausanne
Department Global Health Institute
Lab UPCOL
Street address EPFL SV GHI UPCOL, Station 19
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platforms (1)
GPL17879 Illumina HiSeq 2500 (Mycobacterium tuberculosis)
Samples (12)
GSM3377905 M. tuberculosis H37Rv, control, replicate 1
GSM3377906 M. tuberculosis H37Rv, control, replicate 2
GSM3377907 M. tuberculosis H37Rv, delta mycP1-eccE1, replicate 1
Relations
BioProject PRJNA489657
SRA SRP159843

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119582_CountTable_no-rRNA.txt.gz 102.0 Kb (ftp)(http) TXT
GSE119582_DESeq2_resLFC_delta-mycP1-eccE1_complemented-with-eccE1_vs_Rv-wt.csv.gz 220.3 Kb (ftp)(http) CSV
GSE119582_DESeq2_resLFC_delta-mycP1-eccE1_complemented-with-mycP1-eccE1_vs_Rv-wt.csv.gz 215.5 Kb (ftp)(http) CSV
GSE119582_DESeq2_resLFC_delta-mycP1-eccE1_complemented-with-mycP1_vs_Rv-wt.csv.gz 221.8 Kb (ftp)(http) CSV
GSE119582_DESeq2_resLFC_delta-mycP1-eccE1_vs_Rv-wt.csv.gz 205.3 Kb (ftp)(http) CSV
GSE119582_DESeq2_resLFC_delta-mycP1-eccE1_with-EmptyVector_vs_Rv-wt.csv.gz 212.7 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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