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Series GSE119562 Query DataSets for GSE119562
Status Public on Sep 07, 2018
Title Definition of Natural Killer cell heterogeneity in humans and mice by high-throughput single-cell RNA sequencing
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Natural Killer (NK) cells are innate lymphoid cells (ILCs) involved in antimicrobial and antitumoral responses. Several NK cell subsets have been reported in humans and mice, but their heterogeneity across organs and species remains poorly characterized. We assessed the diversity of human and mouse NK cells by single-cell RNA sequencing on thousands of individual cells isolated from spleen and blood. Unbiased transcriptional clustering revealed two distinct signatures differentiating between splenic and blood NK cells. This analysis at single-cell resolution identified three subpopulations in mouse spleen and four in human spleen, and two subsets each in mouse and human blood. A comparison of transcriptomic profiles within and between species highlighted the similarity of the two major subsets, NK1 and NK2, across organs and species. This unbiased approach provides new insight into the biology of NK cells and establishes a rationale for the translation of mouse studies to human physiology and disease.
 
Overall design Spleen and Blood paired NK cells from 3 individual human brain dead donors (CD45+ CD3– CD14– CD19– CD56+) and 3*5 pooled mice (CD45+ CD3– CD19– NKp46+ NK1.1+) were sorted.
Sample 1 : Frozen live spleen NK cells from donor 1 mixed with fresh live spleen NK cells from mouse group 1.
Sample 2 : Frozen live spleen NK cells from donor 2 mixed with fresh live spleen NK cells from mouse group 2.
Sample 3 : Frozen live spleen NK cells from donor 3 mixed with fresh live spleen NK cells from mouse group 3.
Sample 4 : Frozen live blood NK cells from donor 1 mixed with fresh live blood NK cells from mouse group 1.
Sample 5 : Frozen live blood NK cells from donor 2 mixed with fresh live blood NK cells from mouse group 2.
Sample 6 : Frozen live blood NK cells from donor 3 mixed with fresh live blood NK cells from mouse group 3.
 
Contributor(s) Crinier A, Milpied P, Escalière B, Piperoglou C, Galluso J, Balsamo A, Spinelli L, Cervera-Marzal I, Ebbo M, Girard-Madoux M, Jaeger S, Bollon E, Hamed S, Hardwigsen J, Ugolini S, Vély F, Narni-Mancinelli E, Vivier E
Citation(s) 30413361
Submission date Sep 06, 2018
Last update date Mar 26, 2019
Contact name Bertrand Escaliere
E-mail(s) escaliere@ciml.univ-mrs.fr
Organization name CIML
Lab SU-EV
Street address 163 avenue de Luminy
City Marseille
ZIP/Postal code 13288
Country France
 
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM3377673 human spleen NK cells from donor 1
GSM3377674 human spleen NK cells from donor 2
GSM3377675 human spleen NK cells from donor 3
Relations
BioProject PRJNA489583
SRA SRP159806

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Supplementary file Size Download File type/resource
GSE119562_RAW.tar 18.7 Mb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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