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| Status |
Public on Aug 31, 2019 |
| Title |
DUX4-induced histone variants H3.X and H3.Y mark DUX4 target genes for expression (CUT&RUN) |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We determined the effects of short pulses of DUX4 expression on genome-wide transcription, mimicking the embryonic expression of this gene, using a DUX4-inducible cell culture model of FSHD (MB135iDUX4). We found that a second pulse of DUX4 has a synergistic effect and shows greater induction of DUX4 targets. Knockdown of DUX4-induced histone variants H3.X and H3.Y has no effect on DUX4 targets with a single pulse of DUX4 but eliminates the superinduction seen with a second pulse, showing that these histones are required for this increase in expression.
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| Overall design |
genome-wide incorporation of histone variants H3.X and H3.Y in MB135iDUX4 myoblasts with overnight DUX4 induction, in triplicate, as well as IgG control
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| Contributor(s) |
Resnick R, Wong C, Skene P, Hake S, Henikoff S, van der Maarel S, Tapscott S |
| Citation(s) |
31722199 |
| |
| Submission date |
Sep 04, 2018 |
| Last update date |
Dec 05, 2019 |
| Contact name |
Stephen Tapscott |
| E-mail(s) |
stapscot@fredhutch.org
|
| Organization name |
Fred Hutch Cancer Research Center
|
| Department |
Human Biology
|
| Lab |
Tapscott
|
| Street address |
1100 Fairview N. Ave
|
| City |
Seattle |
| State/province |
WASHINGTON |
| ZIP/Postal code |
98103 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE119403 |
DUX4-induced histone variants H3.X and H3.Y mark DUX4 target genes for expression |
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| Relations |
| BioProject |
PRJNA489182 |
| SRA |
SRP159496 |
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