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Series GSE119253 Query DataSets for GSE119253
Status Public on Jul 17, 2019
Title Expression data from human breast cancer cell line DCIS educated by CAFs
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Emerging evidence supports the hypothesis that multicellular tumor clusters invade and seed metastasis. However, whether tumor-associated stroma induces epithelial-mesenchymal plasticity in tumor cell clusters, to promote invasion and metastasis, remains unknown. We demonstrate herein that carcinoma-associated fibroblasts (CAFs) frequently present in tumor stroma drive the formation of tumor cell clusters composed of two distinct cancer cell populations, one in a highly-epithelial (E-cadherin-high ZEB1-low/neg: E-hi) state and another in a hybrid epithelial/mesenchymal (E-cadherin-low ZEB1-high: E/M) state. The E-hi cells highly express oncogenic cell-cell adhesion molecules, such as carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) and CEACAM6 that associate with E-cadherin, resulting in increased tumor cell cluster formation and metastatic seeding. The E/M cells also retain associations with E-hi cells, which follow the E/M cells leading to collective invasion. CAF-produced stromal cell-derived factor 1 and transforming growth factor-b confer the E-hi and E/M states as well as invasive and metastatic traits via Src activation in apposed human breast tumor cells. Taken together, these findings indicate that invasive and metastatic tumor cell clusters are induced by CAFs via epithelial-mesenchymal plasticity.
 
Overall design The samples included DCISCAF2cy (n=4), DCIScnt2cy (n=4).
 
Contributor(s) Orimo A
Citation(s) 31331982
Submission date Aug 30, 2018
Last update date Aug 07, 2019
Contact name Yoshihiro Mezawa
E-mail(s) y-mezawa@juntendo.ac.jp
Phone 81-(0)3-5802-1039
Organization name Juntendo University
Street address 2-1-1 Hongo, Bunkyo-ku
City Tokyo
ZIP/Postal code 113-8421
Country Japan
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (8)
GSM3362313 DCIScnt2cy rep1
GSM3362314 DCIScnt2cy rep2
GSM3362315 DCIScnt2cy rep3
Relations
BioProject PRJNA488614

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE119253_RAW.tar 33.5 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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