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Series GSE11916 Query DataSets for GSE11916
Status Public on Sep 30, 2009
Title Comparison of TCF hyper-activated and control Huh7 cells - microarray data
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The Wnt signaling pathway is involved in many differentiation events during embryonic development and can lead to tumor formation after aberrant activation of its components. Β-catenin, a cytoplasmic component, plays a major role in the transduction of the canonical wnt/ β-catenin signaling. The aim of this study was to identify novel genes that are regulated by active β-catenin/TCF signaling in hepatocellular carcinoma. We selected and expanded isogenic clones from hepatocellular carcinoma-derived Huh7 cells with high and low β-catenin/TCF activities. We showed that, high TCF activity Huh7 cells lead to bigger and more aggressive tumors when xenografted into nude mice. We used SAGE (Serial Analysis of Gene Expression), genome-wide microarray and in silico promoter analysis in parallel, to compare gene expression between low (basal) and high (transfected) β-catenin/TCF activity clones, those had been xenografted into nude mice. We compared and contrasted SAGE and genome-wide microarray data, in parallel. Finally; after combined analysis, we identified BRI3 and HSF2 as novel targets of Wnt/β-catenin signaling in hepatocellular carcinoma.
Overall design High TCF activity Huh7 cell line (Huh7-S33Y) was compared to control Huh7 cell line (Huh7-Vec) by using 10 ug of total RNA isolated from each sample (15 ug of labeled cRNA was hybridized to the arrays). Triplicates are coming from same total RNA extraction.
Contributor(s) Kavak E, Duru AD, Koman A
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Submission date Jun 27, 2008
Last update date Jan 08, 2019
Contact name Ersen Kavak
Phone 0046707875729
Organization name Bogazici University
Department Molecular Biology and Genetics
Lab AKLAB (Ahmet Koman Lab)
Street address Bogazici Uni. North Campus, North otopark building, 212
City Istanbul
ZIP/Postal code 34342
Country Turkey
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (6)
GSM301104 Control Huh7_Vec#1
GSM301105 Control Huh7_Vec#2
GSM301106 Control Huh7_Vec#3
This SubSeries is part of SuperSeries:
GSE11956 Comparison of TCF hyper-activated and control Huh7 cells
BioProject PRJNA109249

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11916_RAW.tar 24.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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