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| Status |
Public on Apr 18, 2019 |
| Title |
Expression changes in early postnatal mouse sciatic nerve after Schwann cell-specific deletion of the Ep400 chromatin remodeler |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To address the role of INO80/SWR-type remodeling complexes, we deleted Ep400 in developing Schwann cells. Ep400 becomes important for proper Schwann cell development at differentiation. Its absence in Schwann cells leads to a peripheral neuropathy. RNA-Seq studies were performed on sciatic nerve from control mice and mice with a Schwann cell-specific deletion of Ep400 to analyze changes in gene expression. These revealed substantial changes in the expression of genes associated with immune and inflammatory response, myelination-associated lipid metabolism and developmental regulatory genes whose occurrence and action is normally limited to earlier stages of development.
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| Overall design |
Schwann cell mRNA profiles of 9-day old control (ctrl) and Ep400 cko mice were generated using Illumina HiSeq 2500 platform.
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| Contributor(s) |
Fröb F, Wegner M |
| Citation(s) |
31142747 |
| Submission date |
Aug 28, 2018 |
| Last update date |
Jun 21, 2019 |
| Contact name |
Michael Wegner |
| Organization name |
Friedrich-Alexander Universität Erlangen-Nürnberg
|
| Department |
Institut of Biochemistry
|
| Lab |
Michael Wegner
|
| Street address |
Fahrstr. 17
|
| City |
Erlangen |
| State/province |
Bavaria |
| ZIP/Postal code |
91054 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
|
| GSM3359109 |
sciatic nerve Ep400_ctrl P9, biological replicate 1 |
| GSM3359110 |
sciatic nerve Ep400_ctrl P9, biological replicate 2 |
| GSM3359111 |
sciatic nerve Ep400_ctrl P9, biological replicate 3 |
| GSM3359112 |
sciatic nerve Ep400_cko P9, biological replicate 1 |
| GSM3359113 |
sciatic nerve Ep400_cko P9, biological replicate 2 |
| GSM3359114 |
sciatic nerve Ep400_cko P9, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA488240 |
| SRA |
SRP159006 |
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