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| Status |
Public on Aug 25, 2018 |
| Title |
RNA sequencing of FHR1-treated human monocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The plasma protein FHR1 induces release of inflammatory cytokines IL-1β, IL-6, IL-18 or TNFα from blood-derived human monocytes. RNA sequencing was performed from RNA of BSA- or FHR1-treated monocytes from 4 different donors. In response to FHR1, 522 monocytic genes were upregulated (gene ontology enrichment analysis), including 35 inflammation related genes, e.g. TNF. Also, G protein-coupled receptors such as EMR2/ADGRE2 were upregulated in response to FHR1.
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| Overall design |
Blood-derived monocytes were treated with BSA or FHR1, after 4h RNA was isolated. RNA of 4 donors were combined and sequenced.
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| Contributor(s) |
Irmscher S, Halder LD, Skerka C |
| Citation(s) |
31273197, 34795372 |
| |
| Submission date |
Aug 24, 2018 |
| Last update date |
Nov 30, 2021 |
| Contact name |
Christine Skerka |
| E-mail(s) |
christine.skerka@leibniz-hki.de
|
| Phone |
+49 3641 532-1164
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| Organization name |
Leibniz Institute for Natural Product Research and Infection Biology
|
| Department |
Infection Biology
|
| Street address |
Beutenbergstraße 11A
|
| City |
Jena |
| ZIP/Postal code |
07747 |
| Country |
Germany |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA487789 |
| SRA |
SRP158776 |
