Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing
Summary
Identification of Nanog targets by transcriptomic changes of Nanog induction and depletion, identification of Nanog co-binding logic by ChIP-seq of Nanog co-binding factors with and without Nanog depletion. H3K27me3 with Nanog induction in +/- Lif.
Overall design
RNA-seq performed on 44iN +/- Dox, SunTag Nanog & Nanog/Otx2 in +/- Dox +/- Lif. ChIP for Brg1, Esrrb, Sox2, Oct4 in 44iN +/- Dox. ATAC-seq in 44iN +/- Dox. H3K27me3 ChIP-seq in Suntag Nanog +/- Dox +/- Lif.
Less...
More...