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Series GSE11857 Query DataSets for GSE11857
Status Public on Jun 20, 2009
Title Gene expression patterns associated with grapevine resistance to downy mildew mediated by the Rpv1 and Rpv2 genes
Platform organism Vitis vinifera
Sample organism Vitis rotundifolia
Experiment type Expression profiling by array
Summary Grapevine downy mildew caused by the Oomycete Plasmopara viticola is one of the most important diseases affecting Vitis spp. However, all cultivated European grapevine varieties are susceptible to P. viticola and the resistance needs to be introduced from other Vitaceae. Segregating populations derived from Muscadinia rotundifolia, a species closely related to Vitis, lead to the identification and mapping of two resistance genes, named Rpv1 and Rpv2. The macroscopic phenotypes of the resistance mediated by the two loci are different. Rpv2 plants completely inhibit P. viticola sporulation and produce very small necrotic lesions. In contrast, Rpv1 plants allow a rather limited but visible sporulation of P. viticola. The aim of the study is to understand the gene expression changes associated with downy mildew resistance mediated by these two loci. Gene expression patterns after P. viticola inoculation or mock inoculation are compared in incompatible (resistant plants bearing Rpv1 or Rpv2 locus) and compatible (susceptible plants with no resistance loci) interactions, using the Vitis vinifera GeneChip from Affymetrix. In order to limit the effect of the genetic background, the plant material consists in three pools of genotypes called B, C and D, respectively corresponding to partially resistant plants (Rpv1+/Rpv2-), totally resistant plants (Rpv1-/Rpv2+), and susceptible plants (Rpv1-/Rpv2-) derived from a segregating population. Each pool consists in 3 different genotypes. Plant leaf discs were inoculated with P. viticola sporangium suspension (i) or mock-inoculated with water (ni) and analysed 6 hours post-inoculation. For each experimental condition, we performed two biological replicates.

Keywords: normal vs disease comparison
Overall design 3 plant genotypes were analyzed: Rpv1+/Rpv2- (B), Rpv1-/Rpv2+ (C), and Rpv1-/Rpv2- (D). Plants were either inoculated with P. viticola (i) or mock-inoculated with water (ni). Biological replicates were performed for each experimental condition.
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Contributor(s) Balzergue S, Merdinoglu D, Martin-Magniette ML
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Submission date Jun 23, 2008
Last update date Jun 22, 2017
Contact name sandrine balzergue
Organization name INRA
Street address Rue G. Morel
City Beaucouze
ZIP/Postal code 49000
Country France
Platforms (1)
GPL1320 [Vitis_Vinifera] Affymetrix Vitis vinifera (Grape) Genome Array
Samples (12)
GSM299451 B_ni_rep1
GSM299452 B_ni_rep2
GSM299453 B_i_rep1
BioProject PRJNA105659

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11857_RAW.tar 22.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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