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Series GSE118447 Query DataSets for GSE118447
Status Public on Nov 08, 2018
Title Environmental Toxicant Induced Epigenetic Transgenerational Inheritance of Prostate Pathology and Stromal-Epithelial Cell Epigenome and Transcriptome Alterations: Ancestral Origins of Prostate Disease
Organism Rattus norvegicus
Experiment type Methylation profiling by high throughput sequencing
Summary The incidence of prostate disease is increasing, and some of this increase may be attributable to ancestral exposure to environmental toxicants and epigenetic transgenerational inheritance mechanisms. The goal of the current study was to determine the effects that exposure of gestating female rats to vinclozolin has on the epigenetic transgenerational inheritance of prostate disease, and to characterize by what molecular epigenetic mechanisms this has occurred. Results indicate that there were changes in DNA methylation in both cell types.
Overall design Gestating female rats (F0 generation) were exposed to vinclozolin by daily injections during E8-E14 of gestation. F1 generation offspring were bred to produce the F2 generation, which were bred to produce the transgenerational F3 generation. Prostate epithelial and stromal cells were isolated from F3 generation from 20-day old rats, prior to the onset of disease, and used to obtain DNA for analysis. MeDIP-seq was used to characterize the methylation present in the cells.
Contributor(s) Klukovich R, Nilsson E, Sadler-Riggleman I, Beck D, Xie Y, Yan W, Skinner MK
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Submission date Aug 13, 2018
Last update date Nov 08, 2018
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (12)
GSM3330318 pr.epi.c1
GSM3330319 pr.epi.c2
GSM3330320 pr.epi.c3
BioProject PRJNA485687
SRA SRP157581

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE118447_epi.prostate.csv.gz 616.3 Mb (ftp)(http) CSV
GSE118447_str.prostate.csv.gz 621.7 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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