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Series GSE11842 Query DataSets for GSE11842
Status Public on Jun 20, 2008
Title Transgenerational effects of the endocrine disruptor Vinclozolin on the prostate transcriptome and adult onset disease
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary The ability of an endocrine disruptor exposure during gonadal sex determination to promote a transgenerational prostate disease phenotype was investigated in the current study.

METHODS: Exposure of an F0 gestating female rat to the endocrine disruptor vinclozolin during F1 embryo gonadal sex determination promoted a transgenerational adult onset prostate disease phenotype. The prostate disease phenotype and physiological parameters were determined for males from F1 to F4 generations and the prostate transcriptome was assessed in the F3 generation.

RESULTS: Although the prostate in prepubertal animals develops normally, abnormalities involving epithelial cell atrophy, glandular dysgenesis, prostatitis, and hyperplasia of the ventral prostate develop in older animals. The ventral prostate phenotype was transmitted for four generations (F1-F4). Analysis of the ventral prostate transcriptome demonstrated 954 genes had significantly altered expression between control and vinclozolin F3 generation animals. Analysis of isolated ventral prostate epithelial cells identified 259 genes with significantly altered expression between control and vinclozolin F3 generation animals. Characterization of regulated genes demonstrated several cellular pathways were influenced, including calcium and WNT. A number of genes identified have been shown to be associated with prostate disease and cancer, including beta-microseminoprotein (Msp) and tumor necrosis factor receptor superfamily 6 (Fadd).

CONCLUSIONS: The ability of an endocrine disruptor to promote transgenerational prostate abnormalities appears to involve an epigenetic transgenerational alteration in the prostate transcriptome and male germ-line. Potential epigenetic transgenerational alteration of prostate gene expression by environmental compounds may be important to consider in the etiology of adult onset prostate disease.

Keywords: expression analysis, transgenerational changes due to Vinclozolin
Overall design RNA samples from ventral prostate or prostate epithelium from F3 generation control rats are compared to corresponding tissues from F3 generation Vinclozolin treated rats
Web link
Contributor(s) Anway M, Skinner MK
Citation(s) 18220299
Submission date Jun 20, 2008
Last update date Jul 31, 2017
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platforms (1)
GPL1355 [Rat230_2] Affymetrix Rat Genome 230 2.0 Array
Samples (8)
GSM299114 F3-Control Ventral Prostate, replica 1
GSM299115 F3-Control Ventral Prostate, replica 2
GSM299116 F3-Vinclozolin Ventral Prostate, replica 1
BioProject PRJNA105633

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11842_RAW.tar 16.6 Mb (http)(custom) TAR (of CEL)
Raw data provided as supplementary file
Processed data included within Sample table

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