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Series GSE118381 Query DataSets for GSE118381
Status Public on Nov 08, 2018
Title Environmental Toxicant Induced Epigenetic Transgenerational Inheritance of Ovarian Pathology and Granulosa Cell Epigenome and Transcriptome Alterations: Ancestral Origins of PCO and POI
Organism Rattus norvegicus
Experiment type Methylation profiling by high throughput sequencing
Summary Previous studies have shown that exposure to a number of environmental toxicants can promote the epigenetic transgenerational inheritance of ovarian disease, including decreases in the primordial follicle pool of oocytes that are similar to what is seen in POI, and increases in ovarian cysts that are similar to what is seen in PCOS. In the current study, transgenerational changes to the epigenome of ovarian granulosa cells are characterized in F3 generation rats after ancestral vinclozolin or DDT exposures compared to controls. In purified granulosa cells from 20 day old F3 generation females 164 differentially methylated regions (DMRs) (p<1e-06) were found in the F3 generation vinclozolin lineage, and 293 DMRs (p<1e-06) in the DDT lineage, compared to controls.
Overall design Pregnant F0 generation female rats were transiently exposed with vinclozolin, DDT or control vehicle (dimethylsulfoxide, DMSO) from days 8-14 of gestation. The in utero exposed offspring, F1 generation rats, were bred to produce the F2 generation, and similarly F2 generation animals were bred to produce the transgenerational F3 generation. No sibling or cousin crosses were used to avoid inbreeding artifacts. Only the F0 generation rats received the experimental treatments. Granulosa cells were harvested from the ovaries of super-ovulated F3 generation females at 20-22 days of age. Granulosa cells were isolated and analyzed so as to characterize DNA methylation.
Contributor(s) Nilsson E, Klukovich R, Sadler-Riggleman I, Beck D, Xie Y, Yan W, Skinner MK
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Submission date Aug 09, 2018
Last update date Nov 08, 2018
Contact name Michael K Skinner
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (9)
GSM3326030 gran.c1
GSM3326031 gran.c2
GSM3326032 gran.c3
BioProject PRJNA485368
SRA SRP156966

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Supplementary file Size Download File type/resource
GSE118381_ddt.granulosa.csv.gz 703.4 Mb (ftp)(http) CSV
GSE118381_vin.granulosa.csv.gz 705.1 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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