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Series GSE118306 Query DataSets for GSE118306
Status Public on Nov 08, 2018
Title Epigenetic Transgenerational Inheritance of Testis Pathology and Sertoli Cell Epimutations: Generational Origins of Male Infertility
Organism Rattus norvegicus
Experiment type Methylation profiling by high throughput sequencing
Summary Environmental toxicant induced epigenetic transgenerational inheritance has been shown to affect testis pathology and sperm count. Sertoli cells have an essential role in spermatogenesis by providing physical and nutritional support for developing germ cells. The current study was designed to further investigate the transgenerational epigenetic changes in the rat Sertoli cell epigenome that are associated with the onset of testis disease.
 
Overall design Gestating female F0 generation rats were transiently exposed to the toxicants DDT (dichlorodiphenyltrichloroethane) or vinclozolin. The F1 generation offspring were bred to generate the F2 generation which were then bred to the transgenerational F3 generation. The DNA was obtained from purified Sertoli cells isolated from 20-day testis of transgenerational F3 generation rats. Transgenerational alterations in DNA methylation were observed in the Sertoli cells.
 
Contributor(s) Sadler-Riggleman I, Klukovich R, Nilsson E, Beck D, Xie Y, Yan W, Skinner MK
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Submission date Aug 08, 2018
Last update date Nov 08, 2018
Contact name Michael K Skinner
E-mail(s) skinner@mail.wsu.edu
Organization name WSU
Department SBS
Street address Abelson 507
City Pullman
State/province WA
ZIP/Postal code 99163
Country USA
 
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (9)
GSM3324490 sc.c1: Sertoli Cells Control
GSM3324491 sc.c2: Sertoli Cells Control
GSM3324492 sc.c3: Sertoli Cells Control
Relations
BioProject PRJNA485141
SRA SRP156759

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE118306_ddt.sertoli.csv.gz 728.8 Mb (ftp)(http) CSV
GSE118306_vin.sertoli.csv.gz 708.3 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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