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| Status |
Public on Dec 31, 2020 |
| Title |
Pericardial fluid exosome miRNA profiling |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by RT-PCR
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| Summary |
Pericardial sac surrounding the heart contains pericardial fluid (PF), which is rich in exosomes. PF exosomes increase angiogenesis in hypoxic endothelial cells and in animal model of hindlimb ischemia by passing the proangiogenic miRNAs to recipient cells. However, under pathological conditions such as diabetes, exosome cargo composition changes and harmful miRNAs can be transferred to the recipient cells and induce more deleterious effects in target tissues. In order to check cargo composition of different PF exosomes, we used PF exosomes from non-diabetic aortic valve replacement (AVR), mitral valve replacement (MVR), coronary artery bypass grafting (CABG) patients and CABG patients with diabetes.
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| Overall design |
PF samples were collected from non-diabetic aortic valve replacement (AVR), mitral valve replacement (MVR), coronary artery bypass grafting (CABG) patients and CABG patients with diabetes (n=4 for each group) and exosomes were isolated from these samples using a commercially available kit. miRNA profiles of exosomes were determined using Exiqon human V4 microRNA PCR panel I+II.
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| Contributor(s) |
Aday S |
| Citation(s) |
33744469 |
| |
| Submission date |
Aug 03, 2018 |
| Last update date |
Mar 24, 2021 |
| Contact name |
Sezin Aday |
| E-mail(s) |
sezin_aday@yahoo.com
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| Organization name |
University of Pennsylvania
|
| Street address |
210 South 33rd Street, Skirkanich Hall
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| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL22600 |
microRNA Ready-to-Use PCR, Human panel I+II, V4.R |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA484411 |
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