|
| Status |
Public on Aug 03, 2018 |
| Title |
Characterization of endothelial and smooth muscle cells from different canine vessels |
| Organism |
Canis lupus familiaris |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Canine primary endothelial cells (ECs) and vascular smooth muscle cells (vSMCs) were isolated from the vena cava, vena porta and aorta. All tissue sources were derived from three donors for accurate comparison and to reduce inter-animal variation. We used a recently established technique for the isolation of primary ECs with a lesser chance of contamination that can also be used on small vessels. This same vessel was also used for the isolation of vSMCs
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| Overall design |
whole transriptome data of canine endothelial cells and vascular smooth muscle cells produced on Illumina NextSeq500 using a single-end 75 bp configuration
|
| Web link |
https://doi.org/10.3389/fphys.2019.00101
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| |
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| Contributor(s) |
Oosterhoff LA, Kruitwagen HS, van Wolferen ME, van Balkom BW, Mokry M, Lansu N, van den Dungen NA, Penning LC, Spanjersberg TC, de Graaf JW, Veenendaal T, Zomerdijk F, Fledderus JO, Spee B, van Steenbeek FG |
| Citation(s) |
30809157 |
| |
| Submission date |
Aug 02, 2018 |
| Last update date |
Mar 06, 2019 |
| Contact name |
Frank van Steenbeek |
| E-mail(s) |
f.g.vansteenbeek@uu.nl
|
| Phone |
0302532848
|
| Organization name |
Utrecht University
|
| Street address |
Yalelaan 104
|
| City |
Utrecht |
| State/province |
Select State/Province |
| ZIP/Postal code |
3584 CM |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL21400 |
Illumina NextSeq 500 (Canis lupus familiaris) |
|
| Samples (18)
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| Relations |
| BioProject |
PRJNA484120 |
| SRA |
SRP156134 |
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