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| Status |
Public on Jan 18, 2019 |
| Title |
Loss of miR-210 leads to progressive retinal degeneration in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
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| Summary |
miRNAs are small, non-coding RNAs that regulate gene expression post-transcriptionally. We used small RNA sequencing to identify tissue-specific miRNAs in the adult brain, thorax, gut and fat body of Drosophila melanogaster. One of the most brain-specific miRNAs that we identified was miR-210, an evolutionarily highly conserved miRNA implicated in the regulation of hypoxia in mammals. In Drosophila, we show that miR-210 is specifically expressed in sensory organs including photoreceptors. miR-210 knock-out mutants are not sensitive towards hypoxia but show progressive degradation of photoreceptor cells, accompanied by decreased photoreceptor potential, demonstrating an important function of miR-210 in photoreceptor maintenance and survival.
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| Overall design |
Multi-tissue miRNA-seq in wild type D. melanogaster to identify tissue-specific miRNAs. RNA-seq of miR-210 knockout fly heads to identify potential targets.
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| Contributor(s) |
Weigelt CM, Hahn O, Arlt K, Gruhn M, Eßer J, Werner JA, Klein C, Büschges A, Grönke S, Partridge L |
| Citation(s) |
30670478 |
| Submission date |
Aug 01, 2018 |
| Last update date |
Apr 02, 2019 |
| Contact name |
Oliver Hahn |
| E-mail(s) |
ohahn@calicolabs.com
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| Organization name |
Calico Life Sciences, LLC
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| Lab |
Oliver Hahn
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| Street address |
1170 Veterans Blvd
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| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA483990 |
| SRA |
SRP155995 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE118004_Differential_Gene_expression_Analysis_results_mir210KO_vs_WT.txt.gz |
697.3 Kb |
(ftp)(http) |
TXT |
| GSE118004_RNA-seq.Readcount_output.txt.gz |
170.1 Kb |
(ftp)(http) |
TXT |
| GSE118004_miRNA-seq.Readcount_output.txt.gz |
8.7 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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