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| Status |
Public on Aug 21, 2008 |
| Title |
Estrogen- and Myc-regulated genes in MCF-7 breast cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Estrogen-responsive genes were identified by transcript profiling of estrogen-treated MCF-7 breast cancer cells.
The gene expression profile generated after estrogen treatment was compared with that following inducible expression of c-Myc or c-Zip (a deletion mutant of c-Myc that lacks the N-terminal transactivation domains) in clonal MCF-7 cell lines.
Keywords: Single time point
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| Overall design |
RNA was collected in three independent experiments, each including parental MCF-7 cells treated with 17b-estradiol (E2) or ethanol (EtOH), zinc-treated p∆MT-c-Myc cells, zinc-treated p∆MT-c-Zip cells and zinc-treated empty vector (p∆MT) cells. Cells were arrested for 48 h with 10 nM ICI 182780 and then treated for 6 h with either 100 nM E2 or ethanol vehicle, or 75 mM zinc for the stably transfected cell lines.
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| |
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| Contributor(s) |
Musgrove E |
| Citation(s) |
18714337 |
| |
| Submission date |
Jun 16, 2008 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Liz Musgrove |
| E-mail(s) |
e.musgrove@garvan.org.au
|
| Phone |
+61 2 9295 8328
|
| Fax |
+61 2 9295 8321
|
| Organization name |
Garvan Institute of Medical Research
|
| Department |
Cancer Research Program
|
| Street address |
384 Victoria Street, Darlinghurst
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA105991 |
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