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Series GSE117895 Query DataSets for GSE117895
Status Public on Jul 31, 2018
Title Activated CARD11 accelerates germinal center kinetics, promoting mTORC1 and terminal differentiation.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary This scRNA-seq experiment is an integral part of a manuscript with the above title. Our analysis of the scRNA-seq data suggests that activated CARD11 promotes immunoglobulin class-switching in germinal center B cells and generation of IgG1-secreting plasma cells.
 
Overall design Single-cell suspensions were prepared from spleens harvested from mice 5 days post immunization with sheep red blood cells. B cells were enriched using an immunomagnetic negative selection kit. scRNA-seq was performed using the Chromium product suite by 10x Genomics.
 
Contributor(s) Chawla R, Wray-Dutra MN, Trapnell C, James RG, Rawlings DJ
Citation(s) 30127060
Submission date Jul 30, 2018
Last update date Mar 25, 2019
Contact name Raghav Chawla
E-mail(s) rchawla@uw.edu
Organization name University of Washington
Department Department of Genome Sciences
Lab Trapnell Lab
Street address 3720 15th Ave NE
City Seattle
State/province WA
ZIP/Postal code 98195
Country USA
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (2)
GSM3314675 1_D5
GSM3314676 1_aCARD11
Relations
BioProject PRJNA483499
SRA SRP155778

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117895_RAW.tar 3.0 Mb (http)(custom) TAR (of H5)
GSE117895_custom_genome.fa.gz 773.0 Mb (ftp)(http) FA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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