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| Status |
Public on Jul 31, 2018 |
| Title |
Activated CARD11 accelerates germinal center kinetics, promoting mTORC1 and terminal differentiation. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
This scRNA-seq experiment is an integral part of a manuscript with the above title. Our analysis of the scRNA-seq data suggests that activated CARD11 promotes immunoglobulin class-switching in germinal center B cells and generation of IgG1-secreting plasma cells.
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| Overall design |
Single-cell suspensions were prepared from spleens harvested from mice 5 days post immunization with sheep red blood cells. B cells were enriched using an immunomagnetic negative selection kit. scRNA-seq was performed using the Chromium product suite by 10x Genomics.
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| Contributor(s) |
Chawla R, Wray-Dutra MN, Trapnell C, James RG, Rawlings DJ |
| Citation(s) |
30127060 |
| |
| Submission date |
Jul 30, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Raghav Chawla |
| E-mail(s) |
rchawla@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Department of Genome Sciences
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| Lab |
Trapnell Lab
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| Street address |
3720 15th Ave NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA483499 |
| SRA |
SRP155778 |
