|
| Status |
Public on Nov 11, 2018 |
| Title |
Reprogram-Seq: A platform for single-cell combinatorial reprogramming [V] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Reprogram-Seq leverages organ-specific cell atlas data with single-cell perturbation and computational analysis to predict, evaluate, and optimize TF combinations that reprogram a cell type of interest.
|
| |
|
| Overall design |
Focusing on the cardiac system, we performed Reprogram-Seq on P0 mouse heart cells to generate a reference transcriptomic map. Based on the reference map, we selected TF candidates and tests 1000s of TF cocktails for direct lineage conversion by scRNA-Seq.
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| |
|
| Contributor(s) |
Hon G, Munshi N, Duan J |
| Citation(s) |
31216470 |
| |
| Submission date |
Jul 27, 2018 |
| Last update date |
Aug 06, 2019 |
| Contact name |
Gary Hon |
| Organization name |
UT Southwestern
|
| Department |
Department of Obstetrics and Gynecology
|
| Lab |
Hon Lab
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (1) |
| GSM3308816 |
10X_Reprogrammed MEFs, biological replicate 1, technical replicate 1 |
|
| This SubSeries is part of SuperSeries: |
| GSE117795 |
Reprogram-Seq: A platform for single-cell combinatorial reprogramming |
|
| Relations |
| BioProject |
PRJNA483197 |
| SRA |
SRP155521 |
