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Series GSE117666 Query DataSets for GSE117666
Status Public on Jul 16, 2019
Title miRNA expression profiling of the A375 melanoma cell line and its dabrafenib-resistant A375R sub-line.
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Altered expression of miRNAs has been demonstrated in tumor tissue and plasma/serum of cancer patients. miRNAs have been shown to have both diagnostic and prognostic significance and to potentially constitute novel targets and therapeutic agents for cancer treatment. Experimental evidences also support a role of miRNAs in tumor cell response to therapy, including resistance to BRAF inhibitors (BRAFi) in melanoma. In this study, miRNA expression pattern was determined in a BRAFV600E melanoma cell line sensitive to the BRAFi dabrafenib (i.e A375) and in its drug-resistant counterpart (i.e. A375R), derived in vitro by culturing the sensitive cell line with progressively increasing concentrations of dabrafenib (from 8 nM to 1.5 mM).
 
Overall design Total RNA was extracted from the melanoma cell line A375 and its dabrafenib-resistant sub-line A375R, and miRNA expression pattern was determined using Affymetrix GeneChip® miRNA 3.1 microarrays.
 
Contributor(s) Amaro A, Pfeffer U, Caporali S, D'Atri S S
Citation(s) 31227006
Submission date Jul 25, 2018
Last update date Jul 18, 2019
Contact name Adriana Agnese Amaro
E-mail(s) adriana.amaro@hsanmartino.it
Phone +390105558312
Organization name IRCCS Ospedale Policlinico SAN MARTINO - IST - NATIONAL CANCER RESEARCH INSTITUTE
Department Molecular Pathology
Street address Largo Rosanna Benzi 10
City Genoa
ZIP/Postal code 16132
Country Italy
 
Platforms (1)
GPL16384 [miRNA-3] Affymetrix Multispecies miRNA-3 Array
Samples (6)
GSM3305847 A375 Parental, biological rep1
GSM3305848 A375R Dabrafenib-resistant, biological rep1
GSM3305849 A375R Dabrafenib-resistant, biological rep2
Relations
BioProject PRJNA482854

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE117666_RAW.tar 27.1 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table

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