|
| Status |
Public on Jul 26, 2018 |
| Title |
Genetic and Epigenetic Determinants Establish a Continuum of Hsf1 Occupancy and Activity Across the Yeast Genome |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Other
|
| Summary |
We performed ChIP-seq of Hsf1 under non heat shock, 5-minute heat shock and 120 minute heat shock conditions. We used the conditional chemical genetics approach known as “anchor away” (AA) to rapidly inactivate Hsf1. We coupled Hsf1-AA to and nascent RNA seq (NAC)-seq to define the genes whose expression depends on Hsf1 during heat shock.
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| |
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| Overall design |
3 yeast Hsf1 ChIP-seq samples, 4 yeast NET-seq samples
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| |
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| Contributor(s) |
Pincus D |
| Citation(s) |
30605674, 30332327 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| DP5 OD017941 |
Quantitative approaches to reveal the homeostatic control mechanisms of stress re |
WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH |
David Pincus |
| R15 GM128065 |
Chromosomal Conformation and Nuclear Organization of Heat Shock Protein Genes |
LSU HEALTH SCIENCES CENTER - SHREVEPORT |
David Samuel Gross |
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| |
| Submission date |
Jul 25, 2018 |
| Last update date |
Apr 28, 2020 |
| Contact name |
David Pincus |
| E-mail(s) |
pincus@uchicago.edu
|
| Phone |
7737950313
|
| Organization name |
University of Chicago
|
| Street address |
929 E 56th St.
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17342 |
Illumina HiSeq 2500 (Saccharomyces cerevisiae) |
| GPL19756 |
Illumina NextSeq 500 (Saccharomyces cerevisiae) |
|
| Samples (7)
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| Relations |
| BioProject |
PRJNA482832 |
| SRA |
SRP155209 |
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