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Series GSE117621 Query DataSets for GSE117621
Status Public on May 17, 2019
Title Differential expression data between E15.5 outflow tracts of Gata4 G295S mutant embryos and littermate controls
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary E15.5 embryos were micro-disscted from Gata4 G295S mutant mice and littermate controls, RNA was isolated using Norgen total RNA isolation, and libraries were generated with the RNA TruSeq Stranded Total RNA kit. 50 base pair paired end reads were obtained on an illumina high seq 2500. Fastq files were aligned to the mouse genome using STAR aligner. QC was performed using RNASeQC and RSeQC. BAM files were processed using cufflinks pipeline.
Overall design The project aims to assess the differential gene expression at E15.5 between the outflow tracts of Gata4 G295S mutant embryos and wildtype littermate controls.
Contributor(s) LaHaye S, Garg V
Citation(s) 31138536
Submission date Jul 24, 2018
Last update date Jul 09, 2019
Contact name Vidu Garg
Phone 614-355-5710
Organization name Research Institute at Nationwide Children's Hospital
Department Cardiovascular Research
Lab Vidu Garg
Street address 700 Children's Drive WB4221
City Columbus
State/province OH
ZIP/Postal code 43205
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM3305255 Gata4 G295Ski/wt sample 1
GSM3305256 Gata4 G295Ski/wt sample 2
GSM3305257 Gata4 G295Ski/wt sample 3
BioProject PRJNA482668
SRA SRP155044

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Supplementary file Size Download File type/resource
GSE117621_LaHaye_RNAseq_GeneLevel_Results.xlsx 3.4 Mb (ftp)(http) XLSX
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Raw data are available in SRA
Processed data are available on Series record

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