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Series GSE11762 Query DataSets for GSE11762
Status Public on Oct 08, 2008
Title LWD1 and LWD2 in Arabidopsis photoperiod regulation
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary Transcriptome analysis has revealed a light-regulated WD (tryptophan and aspartate)-containing protein, LWD1. LWD1 and LWD2 share greater than 90% amino acid sequence homology. The lack of phenotype changes in the lwd1 or lwd2 single mutant implies that the proteins function redundantly. The lwd1lwd2 double mutant, however, has an early flowering phenotype under both long-day (LD) and short-day (SD) conditions. Functional complementation experiment revealed that LWDs are indeed responsible for the defect in photoperiod sensing in lwd1lwd2 double mutant plants. The expression of LWD1 exhibits a diurnal pattern and peaks before dawn. The period length of oscillator (CCA1, LHY, TOC1 and ELF4) and output (CCR2 and CAB2) genes in the lwd1lwd2 double mutant is significantly shorter than that in wild-type Arabidopsis under free running condition. Under entrainment conditions, the expression phase of oscillator (CCA1, LHY, TOC1 and ELF4) and output (GI, FKF1, CDF1, CO and FT) genes shifts ~3 hr forward in the lwd1lwd2 double mutant. Our data indicated that the early flowering phenotype in lwd1lwd2 plants is contributed by the significant phase shift of CO and, therefore, an increased expression of FT before dusk under SD conditions. Our data imply that LWD1/LWD2 proteins function in close proximity to the circadian oscillators for the regulation of photoperiod sensing.
Overall design Two biological replicates were performed to examine the differential gene expression between wild-type and lwd1lwd2 double mutant. The plants were grown under 12 h L/ 12 h D for 31 days before harvesting their above ground tissues at ZT5-9.
Contributor(s) Wu J, Wang Y, Wu S
Citation(s) 18676661
Submission date Jun 12, 2008
Last update date Aug 28, 2018
Contact name Shu-Hsing Wu
Phone 886-2-27871178
Fax 886-2-27871178
Organization name Institute of Plant and Microbial Biology, Academia Sinica
Street address 128, Sec 2, Academia Road
City Taipei
ZIP/Postal code 11529
Country Taiwan
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (4)
GSM297894 LWD1 and LWD2 in Arabidopsis photoperiod regulation (wt rep 1)
GSM297895 LWD1 and LWD2 in Arabidopsis photoperiod regulation (lwd1lwd2 rep 1)
GSM297896 LWD1 and LWD2 in Arabidopsis photoperiod regulation (wt rep 2)
BioProject PRJNA106061

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11762_RAW.tar 9.2 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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