|
| Status |
Public on Jan 03, 2022 |
| Title |
Distinct microglial transcriptional responses during opioid exposure or neuropathic pain [mouse] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In settings of heightened pain sensitivity, such as following peripheral nerve injury (PNI) or opioid-induced hyperalgesia (OIH), microglia take on an activated phenotype. Functional studies have suggested that microglia activated by PNI or chronic opioids then engage common mechanisms to facilitate pain. Here we conducted RNA sequencing of acutely isolated spinal cord microglia to comprehensively interrogate commonality between PNI and OIH. By combining our results with meta-analysis of published datasets, we identify transcriptional signatures of microglial reactivity that differ between PNI models over time, opioid exposure, or CNS pathology, despite similar histological outcomes. Collectively, these results reveal a discrepancy between histological markers of activation and transcriptional response, and provide a resource of pain-associated microglial transcriptomes that caution against a universal signature of microglia activation.
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| |
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| Overall design |
RNA sequencing of actutely isolated spinal cord microglia following peripheral nerve injury or opioid treatment, or RNA-seq of cultured rat cortical microglia after morphine treatment or TLR agonist treatment
|
| Web link |
https://www.biorxiv.org/content/10.1101/2021.12.29.473853v1
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| |
|
| Contributor(s) |
Sypek E, Scherrer G |
| Citation missing |
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| |
| Submission date |
Jul 18, 2018 |
| Last update date |
Jan 03, 2022 |
| Contact name |
Gregory Scherrer |
| E-mail(s) |
gs25@stanford.edu
|
| Organization name |
Stanford University
|
| Department |
Anesthesiology
|
| Street address |
1050 Arastradero Rd
|
| City |
Palo Alto |
| State/province |
CA |
| ZIP/Postal code |
94304 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL16417 |
Illumina MiSeq (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE117321 |
Distinct microglial transcriptional responses during opioid exposure or neuropathic pain |
|
| Relations |
| BioProject |
PRJNA481755 |
| SRA |
SRP154357 |
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