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| Status |
Public on Jul 16, 2021 |
| Title |
Genome-wide DNA methylation change due to H19 knockdown and TET3 knockdown in UtLM |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We used Methyl-MiniSeq platform from Zymo Research company to identify genome-wide methylation changes affected by H19 knockdown and TET3 knockdown in human UtLM. Following H19 and TET3 knockdown, we observed extensive genome-wide methylation pattern changes relative to siCon cells, with some genes showing increased methylation, others showing decreased methylation, and a third group with no significant change.
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| Overall design |
human UtLM were transfected with either control siRNA, siH19 or siTET3. 48h later, cellular genomic DNA was extracted and subjected to genome-scale DNA methylation mapping using the platform of an improved version of Reduced Representation Bisulfite Sequencing (RRBS).
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| Contributor(s) |
Giang K, Chung H, Sun X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jul 16, 2018 |
| Last update date |
Jul 17, 2021 |
| Contact name |
Ying Jiang |
| E-mail(s) |
ying.jiang@yale.edu
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| Organization name |
Department of Obstetrics, Gynecology & Reproductive Sciences
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| Street address |
310 Cedar Street
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| City |
New Haven |
| ZIP/Postal code |
06510 |
| Country |
USA |
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| Platforms (1) |
| GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA481374 |
| SRA |
SRP153995 |
