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Status |
Public on Jul 16, 2021 |
Title |
Genome-wide DNA methylation change due to H19 knockdown and TET3 knockdown in UtLM |
Organism |
Homo sapiens |
Experiment type |
Methylation profiling by high throughput sequencing
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Summary |
We used Methyl-MiniSeq platform from Zymo Research company to identify genome-wide methylation changes affected by H19 knockdown and TET3 knockdown in human UtLM. Following H19 and TET3 knockdown, we observed extensive genome-wide methylation pattern changes relative to siCon cells, with some genes showing increased methylation, others showing decreased methylation, and a third group with no significant change.
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Overall design |
human UtLM were transfected with either control siRNA, siH19 or siTET3. 48h later, cellular genomic DNA was extracted and subjected to genome-scale DNA methylation mapping using the platform of an improved version of Reduced Representation Bisulfite Sequencing (RRBS).
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Contributor(s) |
Giang K, Chung H, Sun X |
Citation missing |
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Submission date |
Jul 16, 2018 |
Last update date |
Jul 17, 2021 |
Contact name |
Ying Jiang |
E-mail(s) |
ying.jiang@yale.edu
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Organization name |
Department of Obstetrics, Gynecology & Reproductive Sciences
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Street address |
310 Cedar Street
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City |
New Haven |
ZIP/Postal code |
06510 |
Country |
USA |
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Platforms (1) |
GPL18460 |
Illumina HiSeq 1500 (Homo sapiens) |
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Samples (3) |
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Relations |
BioProject |
PRJNA481374 |
SRA |
SRP153995 |